Myofibroblastic stromal reaction in carcinoma of the breast: variations of collagenous matrix and structural glycoproteins (original) (raw)

Summary

The matrix of mammary dysplasia, noninvasive ductal carcinoma, and invasive lobular and ductal carcinoma was analyzed by indirect immunofluorescence using antibodies to types I, proIII, III, and IV collagens, and laminin and fibronectin. Types proIII and III collagens were present in increased amounts in invasive carcinomas and were most abundant in the “young” edematous mesenchyme, areas corresponding to the peripheral invasive cellular front. Type I collagen was distributed throughout the matrix of invasive carcinomas but was most prominent within the central sclerotic zone of the neoplasms. Mammary dysplasia and noninvasive ductal carcinomas showed a uniform fibrillar and granular distribution of all types of collagen. In all but two cases of invasive carcinoma, staining with anti-laminin and anti-type IV collagen demonstrated the loss of basement membranes around tumor cells. In contrast, fluorescence pattern in noninvasive ductal carcinoma and dysplasia revealed an intact basement membrane. The distribution of fibronectin was similar to types proIII and III collagen.

These findings support and extend our previous studies which suggested an analogy between the dynamics of matrix changes in granulation tissue and invasive carcinomas. These data also strengthen the concept that the myofibroblast could be a pivotal cell involved in the synthesis and redistribution of matricial proteins. The loss of basement membrane in invasive carcinomas appears to be an initial step for inducing the matricial alterations.

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Authors and Affiliations

1. Service de pathologie, Hôtel-Dieu de Québec et départment de pathologie, Faculté de médecine, Université Laval, 11 Côte du Palais, G 1 R 2J 6, Québec, Canada
   Réal Lagacé
2. Laboratoire de pathologie cellulaire du foie, CNRS, ERA 189, Institut Pasteur, Lyon, France
   Jean-Alexis Grimaud
3. Département de pathologie, Hôtel-Dieu de Montréal et département de pathologie, Université de Montréal, Montréal, Québec, Canada
   Walter Schürch
4. Department of Pathology, The Montreal Children's Hospital, McGill University, Montréal, Québec, Canada
   Thomas A. Seemayer

Authors

1. Réal Lagacé
2. Jean-Alexis Grimaud
3. Walter Schürch
4. Thomas A. Seemayer

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Lagacé, R., Grimaud, JA., Schürch, W. et al. Myofibroblastic stromal reaction in carcinoma of the breast: variations of collagenous matrix and structural glycoproteins.Vichows Archiv A Pathol Anat 408, 49–59 (1985). https://doi.org/10.1007/BF00739962

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• Accepted: 08 July 1985
• Issue date: January 1985
• DOI: https://doi.org/10.1007/BF00739962

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