Analysis of phylogenetic relationships in the Triticeae tribe using RFLPs (original) (raw)
Abstract
The use of restriction fragment length polymorphisms in combination with other approaches is very useful for the reconstruction of evolutionary events revealing phylogenetic relationships. A set of 21 cDNA probes hybridizing to different chromosome arms in hexaploid wheat was used in a series of experiments designed to estimate the phylogenetic relationships among and within 16 species of the Triticeae tribe. A high degree of polymorphism was found both between and within the species examined. The RFLP data were used to generate a cladogram and a phenogram in order to compare the two different methods of constructing phylogenetic trees. The results of both methods were consistent with each other and with the general taxonomic information provided by earlier morphological studies, meiotic pairing analysis, isozyme tests, and sequence alignment in the_Ter_,NOR, and_5s_ DNA loci. In addition, several correlations were found between the geographical origin of accessions from the same species and their phylogenetic relationships as shown by the cladogram and phenogram.
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Authors and Affiliations
- USDA-ARS, Plant Genetics Research Unit and Plant Science Unit, University of Missouri, 65211, Columbia, MO, USA
J. V. Monte & J. P. Gustafson - CSIRO, Division of Tropical Crops and Pastures, Cunningham Laboratory, 306 Carmody Road, 4067, St. Lucia, Queensland, Australia
C. L. McIntyre
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- J. V. Monte
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Communicated by J. W Snape
This paper reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by the USDA or the University of Missouri.
This paper is a contribution of the U.S. Department of Agriculture, Agricultural Research Service, and Missouri Agricultural Experimental Station, Journal Series No. 11706.
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Monte, J.V., McIntyre, C.L. & Gustafson, J.P. Analysis of phylogenetic relationships in the Triticeae tribe using RFLPs.Theoret. Appl. Genetics 86, 649–655 (1993). https://doi.org/10.1007/BF00838722
- Received: 20 July 1992
- Accepted: 09 December 1992
- Issue Date: June 1993
- DOI: https://doi.org/10.1007/BF00838722