Characterization of serum-independent polymorphonuclear leukocyte chemotactic factors produced byPropionibacterium acnes (original) (raw)

Abstract

The size and production of polymorphonuclear leukocyte (PMN) chemotactic factors by_Propionibacterium acnes_ has been studied. All eight strains of_P. acnes_ which were tested liberated PMN chemotactic factors in their growth culture media. The factor(s) produced by one strain, 6919, were studied in greater depth. The PMN response was proportional to the dose of culture supernatant and chemotactic activity increased with the duration of_P. acnes_ culture. The neutrophil migration towards culture supernatants was chemotactic, with a slight stimulation of random migration by 72-h supernatants. The size of the chemotactic molecules was studied through dialysis, ultrafiltration, and Sephadex G-25 chromatography of 72-h supernatants. Low-molecular-weight chemotactic factors were found to be predominant by each method of size determination.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Webster, G. F., C.-C. Tsai, andJ. J. Leyden, 1979. Neutrophil lysosomal release in response to_Propionibacterium acnes_.J. Invest. Dermatol. 72:209.
    Google Scholar
  2. Massey, A., J. F. Mowbray, andW. C. Noble, 1978. Complement activation by_Corynebacterium acnes_.Br. J. Dermatol 98:583–584.
    Google Scholar
  3. Webster, G. F., J. J. Leyden, M. E. Norman, andU. R. Nilsson. 1978. Complement activation in acne vulgaris, in vitro studies with_Propionibacterium acnes_ and_Propionibacterium granulosum_.Infect. Immun. 22:523–529.
    Google Scholar
  4. Webster, G. F., J. J. Leyden, andU. R. Nilsson. 1979. Complement activation in acne vulgaris; comsumption of complement by comedones.Infect. Immun. 26:183–186.
    Google Scholar
  5. Gould, D. J., W. J. Cunliffe, andK. T. Holland. 1977. Chemotaxis and acne.J. Invest. Dermatol. 68:251.
    Google Scholar
  6. Lee, W. L., K. Sunthralioam, S. M. Fikrig, andA. R. Shalita. 1977. Neutrophil chemotaxis by_P. acnes_.Clin. Res. 25:283A.
    Google Scholar
  7. Tucker, S. B., R. S. Rodgers III, R. K. Winklemann, andR. E. Jordan. 1977. Inflammation in acne vulgaris. Mechanisms.J. Invest. Dermatol. 68:237.
    Google Scholar
  8. Lee, W. L., andA. R. Shalita. 1978. Neutrophil chemotaxis by_P. acnes_ and its inhibition by antibiotics.J. Invest. Dermatol. 70:219.
    Google Scholar
  9. Puhvel, S. M. andM. Sakamoto. 1978. The chemoattractant properties of comedonal components.J. Invest. Dermatol. 71:324–329.
    Google Scholar
  10. Worthington Biochemical Corporation. 1972. Worthington Enzyme Manual, pp. 7–10. Freehold, New Jersey.
    Google Scholar
  11. Wilkinson, P. C. G. J. O'neill, andK. G. Wapshaw. 1973. Role of anaerobic coryneforms in specific and non-specific immunological reactions, 11: Production of a chemotactic factor specific for macrophages. Immunology.24:997–1009.
    Google Scholar
  12. Schiffman, E., H. V. Showell, B. A. Corcoran, P. A. Ward, E. Smith, andE. L. Becker. 1975. The isolation and partial characterization of neutrophil chemotactic factors from_Escherichia coli_.J. Immunol. 114:1831–1841.
    Google Scholar
  13. Ward, P. A., I. H. Lepow, andL. J. Newman. 1968. Bacterial factors chemotactic for polymorphonuclear leukocytes.Am. J. Pathol. 52:725–736.
    Google Scholar
  14. Yoshida, T., S. Cohen, P. E. Bigazzi, T. Kurasuji, andA. Amsden. 1975. Inflammatory mediators in culture filtrates of_Escherichia coli_.Am. J. Pathol. 81:389–400.
    Google Scholar
  15. Puhvel, S. M., I. K. Huffman, R. M. Reisner, andT. H. Sternberg. 1967. Dermal hypersensitivity of patients with acne vulgaris to_Corynebacterium acnes_.J. Invest. Dermatol. 49:154–158.
    Google Scholar

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, School of Dental Medicine, University of Pennsylvania, 19104, Philadelphia, Pennsylvania
    Guy F. Webster
  2. Department of Dermatology, Duhring Laboratories, School of Medicine, University of Pennsylvania, 19104, Philadelphia, Pennsylvania
    James J. Leyden

Authors

  1. Guy F. Webster
  2. James J. Leyden

Rights and permissions

About this article

Cite this article

Webster, G.F., Leyden, J.J. Characterization of serum-independent polymorphonuclear leukocyte chemotactic factors produced by_Propionibacterium acnes_.Inflammation 4, 261–269 (1980). https://doi.org/10.1007/BF00915027

Download citation

Keywords