Life cycle ofHepatozoon mehlhorni. sp. nov. in the viperEchis carinatus and the mosquitoCulex pipiens (original) (raw)
Abstract
Hepatozoon mehlhori sp. nov. and its developmental stages from the tissues of the Egyptian viper_Echis carinatus_ and the mosquito_Culex pipines_ are described. The erythrocytic parasites were differentiated into the small form (trophozoite) measuring 14.5±0.6×4±0.12 μm and the mature form (gametocyte) measuring 17.2±1.6×5.4±0.5μm. Merogony took place in the pulmonary endothelial cells and in the parenchyma cells of the liver and spleen of the infected vipers. Two types of meront were found. The large meronts (macromeronts) were 30.2±1.73×22.6±1.2 μm in size and yielded 16–40 (average 28) micromerozoites measuring 17.2±0.7×5±0.15 μm. The small, meronts (micromeronts) measured 18.2±0.6×13.5±0.5 μm and yielded 2–14 (average, 8) macromerozoites that were 15.1±0.12×6.2±0.8 μm in size. After syzygy in the haemocoel of the mosquito, the microgamont produced four uniflagel-late microgametes (6.4±0.3×4.5±0.5 μm in size, with a short flagellum measuring 3.2±0.1 μm); on the 3rd day post-infection (p.i.)., one of these fertilized the macrogamete, giving rise to the zygote. The oocyst developed from the zygote on the 5th day p.i. and measured 135±2.6×120±1.8 μm. About 11–60 (average, 35) sporoblasts were formed by centripetal invaginations from each oocyst on the 8th day p.i. and developed into sporocysts on the 14th day p.i. Inside each sporocyst, 5–12 (average, 8) sporozoites, each measuring 12.6±1.2×4.1±0.3 μm, developed on the 16th day p.i. According to the above-mentioned characteristics the parasite was recorded as being a new species and was named_Hepatozoon mehlhorni_. Experimental transmission was accomplished by i.p. inoculation of the infectious stages (sporozoites) into uninfected vipers and led to the appearance of blood stages at 4–6 weeks p.i.
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Abbreviations
BLC :
Blood capillary
DMS :
developing merozoites
DSP :
developing sporoblasts
E :
erythrocyte
F :
flagellum
HC :
host cell
HN :
host nucleus
M :
Meront
MA :
macrogamont/macrogamete
MG :
Microgamete
MIG :
microgamont
MS :
merozoites
N :
nucleus
NG :
micleus of the microgamete
OC :
oocyst
P :
erythrocytic parasite
PV :
Parasitophorous vacuole
SP :
sporoblast (s)
SPC :
sporocyst (s)
SPR :
sporozoite (s)
ZY :
zygote (young oocyst)
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Authors and Affiliations
- Zoology Department, Faculty of Science, Cairo University, Giza, Egypt
A. -R. Bashtar, F. A. Abdel-Ghaffar & M. A. Shazly
Authors
- A. -R. Bashtar
- F. A. Abdel-Ghaffar
- M. A. Shazly
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Bashtar, A.R., Abdel-Ghaffar, F.A. & Shazly, M.A. Life cycle of_Hepatozoon mehlhorni._ sp. nov. in the viper_Echis carinatus_ and the mosquito_Culex pipiens_.Parasitol Res 77, 402–410 (1991). https://doi.org/10.1007/BF00931635
- Accepted: 19 January 1991
- Issue date: May 1991
- DOI: https://doi.org/10.1007/BF00931635