Plasmodium falciparum andP. berghei: detection of sporozoites and the circumsporozoite proteins in the saliva ofAnopheles stephensi mosquitoes (original) (raw)

Abstract

Sporozoites and free circumsporozoite (CS) protein were stained immunoenzymatically in 1-min saliva samples collected from_Anopheles stephensi_ mosquitoes infected with either_Plasmodium berghei_ or_P. falciparum_. The number of sporozoites in 1-min saliva-streak samples significantly increased as the salivary gland index rose from 3+ to 4+. For_P. berghei_-infected mosquitoes from which saliva had been collected before 30 days postfeed, the median sporozoite counts for 3+ and 4+ gland indexes were 4.5 and 116, respectively. For_P. falciparum_-infected mosquitoes, the median counts obtained in two experiments were 4.5 and 14.5 (3+) and 97 and 107 (4+), respectively. The frequency of sporozoite detection in the saliva of mosquitoes containing <100 salivary-gland sporozoites was low (0.1), whereas that in the saliva of mosquitoes with >100 sporozoites was high (0.96). In highly infected 4+_P. berghei_-infected mosquitoes from which saliva had been collected after 30 days postinfection, both the volume of saliva collected and the median number of sporozoites recovered decreased significantly.

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Authors and Affiliations

1. Department of Entomology, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA
   Claudia F. Golenda & Imogene Schneider
2. Department of Biometrics, Walter Reed Army Institute of Research, 20307-5100, Washington, DC, USA
   Robert Burge

Authors

1. Claudia F. Golenda
2. Robert Burge
3. Imogene Schneider

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Golenda, C.F., Burge, R. & Schneider, I. Plasmodium falciparum and_P. berghei_: detection of sporozoites and the circumsporozoite proteins in the saliva of_Anopheles stephensi_ mosquitoes.Parasitol Res 78, 563–569 (1992). https://doi.org/10.1007/BF00936453

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• Accepted: 11 May 1992
• Issue date: July 1992
• DOI: https://doi.org/10.1007/BF00936453

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