Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody (original) (raw)

Summary

Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immuno-fluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3.

Article PDF

References

  1. Barthold SW, Smith AL (1984) Mouse hepatitis virus strain-related patterns of tissue tropism in suckling mice. Arch Virol 81: 103–112
    Google Scholar
  2. Buchmeier MJ, Lewicki HA, Talbot PJ, Knobler RL (1984) Murine hepatitis virus-4 (Strain JHM)-induced neurologic disease is modulated_in vivo_ by monoclonal antibody. Virology 132: 261–270
    Google Scholar
  3. Cainelli-Gebara VC (1986) Production et caractérisation d'anticorps monoclonaux contre le virus de l'hépatite murine type 3. MSc Dissertation, Université du Québec, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada
    Google Scholar
  4. Fleming SO, Stohlman SA, Harmon RC, Lai MMC, Frelinger JA, Weiner LP (1983) Antigenic relationships of murine coronaviruses: analysis using monoclonal antibodies to JHM (MHV-4) virus. Virology 131: 296–307
    Google Scholar
  5. Lamontagne L, Dupuy J-M (1984) Persistent infection with mouse hepatitis virus type 3 in mouse lymphoid cell line. Infect Immun 44: 716–723.
    Google Scholar
  6. Lefrancois L (1984) Protection against lethal viral infection by neutralizing and non-neutralizing monoclonal antibodies: distinct mechanisms of action_in vivo_. J Virol 51: 208–214
    Google Scholar
  7. Levy-Leblond E, Oth D, Dupuy J-M (1979) Genetic study of mouse sensitivity to mouse hepatitis type 3 infection: influence of the H-2 complex. J Immunol 122: 1359–1362
    Google Scholar
  8. Lussier G, Descôteaux J-P (1986) Prevalence of natural virus infections in laboratory mice and rats used in Canada. Anim Sci 36: 145–148
    Google Scholar
  9. Nakanaga K, Yamanouchi K, Fujiwara K (1986) Protective effect of monoclonal antibodies on lethal mouse hepatitis virus infections in mice. J. Virol 59: 168–171
    Google Scholar
  10. Rawls WE, Tompkins WAF (1975) Destruction of virus-infected cells by antibody and complement. In: Notkins AL (ed) Viral immunology and immuno-pathology. Academic Press, New York, pp 99–111
    Google Scholar
  11. Taguchi F, Makino S, Fujiwara K (1982) Antigenic differentiation of mouse hepatitis viruses by neutralization test. Microbiol Immunol 26: 741–745
    Google Scholar
  12. Talbot PJ, Salmi AA, Knobler RL, Buchmeier MJ (1984) Topographical mapping of epitopes on the glycoproteins of murine hepatitis virus 4 (JHM): correlation with biological activities. Virology 132: 250–260
    Google Scholar
  13. Talbot P, Buchmeier MJ (1985) Antigenic variation among murine coronaviruses: evidence for polymorphism on the peplomer glycoprotein E2. Virus Res 2: 317–328
    Google Scholar
  14. Towbin H, Stachelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354
    Google Scholar
  15. Wege H, Stephenson JR, Koga M, Wege H, ter Meulen V (1981) Genetic variation of neurotropic and non-neurotropic murine coronaviruses. J Gen Virol 54: 67–74
    Google Scholar
  16. Wege H, Siddel ST, ter Meulen V (1982) The biology and pathogenesis of coronaviruses. Curr Top Microbiol Immunol 99: 165–200
    Google Scholar
  17. Wege H, Dorries R, Wege H (1984) Hybridoma antibodies to the murine coronavirus JHM: characterization of epitopes on the peplomer protein (E2). J Gen Virol 65: 1931–1942
    Google Scholar
  18. Yewdell JW, Frank E, Gerhard W (1981) Expression of influenza A virus internal antigens on the surface of infected P815 cells. J Immunol 126: 1814–1819
    Google Scholar

Download references

Author information

Author notes

  1. V. Cainelli-Gebara
    Present address: Laboratoria Especial de Immunologia Viral, Instituto Butantan, S~ao Paulo, Brazil

Authors and Affiliations

  1. Centre de recherche en virologie, Institut Armand-Frappier, Ville de Laval, Québec, Canada
    J. Lecomte, P. J. Talbot & G. Lussier
  2. Centre de recherche en immunologie, Institut Armand-Frappier, Ville de Laval, Québec, Canada
    V. Cainelli-Gebara, G. Mercier, S. Mansour & D. Oth

Authors

  1. J. Lecomte
    You can also search for this author inPubMed Google Scholar
  2. V. Cainelli-Gebara
    You can also search for this author inPubMed Google Scholar
  3. G. Mercier
    You can also search for this author inPubMed Google Scholar
  4. S. Mansour
    You can also search for this author inPubMed Google Scholar
  5. P. J. Talbot
    You can also search for this author inPubMed Google Scholar
  6. G. Lussier
    You can also search for this author inPubMed Google Scholar
  7. D. Oth
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Lecomte, J., Cainelli-Gebara, V., Mercier, G. et al. Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody.Archives of Virology 97, 123–130 (1987). https://doi.org/10.1007/BF01310740

Download citation

Keywords