Spread of Herpes simplex virus type-1 (Miyama +GC strain) to the central nervous system after intraperitoneal inoculation: the role of the myenteric plexus of the gut (original) (raw)

Summary

The pathways taken by the HSV-1 virus after intraperitoneal (i.p.) inoculation were studied in 5-week old male C3H/HeN mice injected with 1×104 PFU (100 LD50) or 5×105 PFU (5000 LD50) of HSV-1 (Miyama +GC strain).

At the higher dosage (5×105 PFU), HSV-1 began replicating in the adrenal from the first day, then in the gut and thoracic portion of the spinal cord by the third day, and in the brainstem by the fourth day, as shown by the titers of the virus in these organs. By immunoperoxidase staining HSV-1 was localized in a necrotic area of the adrenal, the myenteric plexus of the gut, the intermediolateral columns of the thoracic cord, and the vagus nerve nuclei of the medulla oblongata.

In the low dose mice (1×104 PFU), HSV-1 was not isolated from the adrenal or thoracic segment of the spinal cord from the time of inoculation until the time of death. It was, however, isolated from the gut on days 4–6 and from the brainstem by day 5. HSV-1 was never isolated from the blood of either group at any time.

The localizations of viral replication suggest that in the mice inoculated with 1×104 PFU, HSV-1 spreads to the brainstem via the vagal nerves after replication in the myenteric plexus of the gut. In the mice given the higher dose, localizations suggest not only the above route, but also that the virus spread to the intermediolateral columns of the spinal cord after replicating in the adrenal.

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References

  1. Beswick TS (1960) Further observations on experimental herpes simplex infection in baby mouse. J Pathol Bacteriol 79: 69–76
    Google Scholar
  2. Cooke BT, Hurst EW, Swan C (1942) Routes of entry into the nervous system of viruses introduced into the bloodstream. Aust J Exp Biol Med Sci 20: 130–138
    Google Scholar
  3. Corey C, Spear PG (1986) Infections with herpes simplex viruses. N Engl J Med 314: 686–691
    Google Scholar
  4. Doerr R, Vochting K (1920) Etudes sur le virus de l'herpe's fébrile. Rev Gen Ophthalmol 34: 409–421
    Google Scholar
  5. Field EJ (1952) Pathogenesis of herpetic encephalitis following corneal and masseteric inoculation. J Pathol Bacteriol 64: 1–11
    Google Scholar
  6. Friedmann U (1943) Permeability of the blood-brain barrier to neurotropic viruses. Arch Pathol 35: 912–934
    Google Scholar
  7. Goodpasture EW, Teague O (1923) Transmission of the virus of herpes febrilis along nerves in experimentally infected rabbits. J Med Res 44: 139–183
    Google Scholar
  8. Goodpasture EW (1925) The axis-cylinders of the peripheral nerves as portals of the entry to the central nervous system for the virus of herpes simplex in experimentally infected rabbits. Am J Pathol 1: 11–27
    Google Scholar
  9. Harada Yu, Kurokawa E, Endo H, Kimura T, Motoo S, Sugawara Yu, Irie H, Ito K, Shimizu Yo, Kumagai K (1988) Growth inhibition of herpes simplex virus by OK-432 in mouse organs. Igakuno Ayumi 145: 61–62 (English abstract)
    Google Scholar
  10. Hill TJ, Yirrell DL, Blyth WA (1986) Infection of the adrenal gland as a route to the central nervous system after viraemia with herpes simplex virus in the mouse. J Gen Virol 67: 309–320
    Google Scholar
  11. Irie H, Mori W, Harada Yu, Kurokawa E, Yamada H, Nii S (1986) Replication of herpes simplex virus in the adrenal of mouse. Igakuno Ayumi 137: 309–310 (in Japanese)
    Google Scholar
  12. Irie H, Harada Yu, Kurokawa E, Saito M, Sugawara Yu, Ohami H, Mori W (1987) Early adrenal infection by herpes simplex virus type-1 (Miyama +GC strain): special reference to inoculation dose and spread from the adrenal to the central nervous system. Virchows Arch [Cell Pathol] 53: 325–331
    Google Scholar
  13. Johnson RT (1964) The pathogenesis of herpes virus encephalitis I. Virus pathways to the nervous system of suckling mice demonstrated by fluorescent antibody staining. J Exp Med 119: 343–356
    Google Scholar
  14. Johnson RT, Mims CA (1968) Pathogenesis of viral infections of the nervous system. N Engl J Med 278: 23–30
    Google Scholar
  15. Kobayashi S, Ohashi T, Fujita T, Nakao K, Yoshimasa T, Imura H, Mochizuki T, Yanaihara C, Yanaihara N, Verhofstadt AAJ (1983) An immunohistochemical study on the co-storage of Met-enkephalin-Arg6-Ply7-Leu8 and Met-enkephalin-Arg6-Phe7 with adrenaline and/or noradrenaline in the adrenal chromaffin cells of the rat, dog, and cat. Biomed Res 4: 433–442
    Google Scholar
  16. Kobayashi S, Ohashi T, Uchida T, Nakao K, Imura H, Yanaihara N, Verhofstadt AAJ (1984) Co-storage of adrenaline and noradrenaline with Met-enkephaline-Arg6-Gly7-Leu8 and Met-enkephaline-Arg6-Gly7-Leu8 and Met-enkephaline Arg6-Phe7 in chromaffin cells of hamster adrenal medulla. Arch Histol Jap 47: 319–336
    Google Scholar
  17. Kristensson K, Vahlne A, Persson A, Lycke E (1978) Neural spread of herpes simplex virus type 1 and 2 in mice after corneal or subcutanous (footpad) inoculation. J Neurol Sci 35: 331–340
    Google Scholar
  18. Lascano EF, Berria MI (1980) Histological study of the progression of herpes simplex virus in mice. Arch Virol 64: 67–79
    Google Scholar
  19. Levaditi C, Hornes G, Harber P (1935) Virulence de l'ultravirus herpetique administre par voies nasale de digestive. Mechanisme de sa neutroprobasie (1) centripede. Ann Inst Pasteur (Paris) 54: 389–420
    Google Scholar
  20. Nachigal M, Gaulfield JB (1984) Early and late pathogenic changes in the adrenal glands of mice after infection with herpes simplex Type 1. Am J Pathol 115: 175–185
    Google Scholar
  21. Nii S, Kamahora J (1961) Studies on the growth of newly isolated herpes simplex virus in vitro. Biken J 4: 75–96
    Google Scholar
  22. Potratz D, Brake B, Dienes HP, Schultz THF, Hosp M, Dierich MP, Falke D (1986) Herpes simplex virus type 1 and 2 in the adrenal glands: replication and histopathology. Arch Virol 90: 207–222
    Google Scholar
  23. Rabin ER, Jenson AB, Melnick JL (1968) Herpes simplex virus in mice. Electron microscopy of the neural spread. Science 162: 126–127
    Google Scholar
  24. Ranson SW, Clark SL (1966) The anatomy of the nervous system. Its development and function, 10th edn. Saunders, London, pp 150–165
    Google Scholar
  25. Slavin HE, Berry GP (1943) Studies on herpetic infection in mice. II. The pathways of intranasal instillation of virus in the suckling mice. J Exp Med 78: 315–320
    Google Scholar
  26. Smith W (1931) Lesions of the adrenal glands of rabbits caused by infection with herpes virus. J Pathol Bacteriol 34: 439–503
    Google Scholar

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Authors and Affiliations

  1. Division of Pathology, Kanto Chuo Hospital, Japan
    H. Irie
  2. Applied Research Laboratories, Chugai Pharmaceutical Co., Ltd., Japan
    Yu. Harada, H. Yoshihashi, T. Kimura, M. Kojima, M. Kataoka, M. Saito & Yu. Sugawara
  3. Department of Pathology, Faculty of Medicine, University of Tokyo, Tokyo, Japan
    W. Mori

Authors

  1. H. Irie
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  2. Yu. Harada
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  3. H. Yoshihashi
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  4. T. Kimura
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  5. M. Kojima
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  6. M. Kataoka
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  7. M. Saito
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  8. Yu. Sugawara
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  9. W. Mori
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Irie, H., Harada, Y., Yoshihashi, H. et al. Spread of Herpes simplex virus type-1 (Miyama +GC strain) to the central nervous system after intraperitoneal inoculation: the role of the myenteric plexus of the gut.Archives of Virology 105, 247–257 (1989). https://doi.org/10.1007/BF01311361

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