Neurogenic spread of Semliki forest virus in mice (original) (raw)

Summary

We present evidence that in SFV-infected mice the virus invades the brain via nervous tracts, and that in the early stage of infection of the brain itself dissemination is restricted to neurons. After intranasal (i.n.) application, migration along the neuronal route appeared to be the principle mechanism of spread irrespective of the virulence of the strain used. After subcutaneous (s.c.) infection, neuronal spread could also be demonstrated, however the pattern and time course of brain invasion were different.

The LD50 of strain Hd depended significantly on the route of virus application: compared with the i.n. route, its value increased after s.c. infection. This increase was correlated with high plasma interferon induction and natural killer cell activation. In contrast, these activities were virtually absent during the early course after i.n. infection.

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References

  1. Armstrong JA (1971) Semi-micro dye-binding assay for rabbit interferon. Appl Microbiol 21: 723–725
    Google Scholar
  2. Atkins GJ, Sheahan BJ, Dimmock NJ (1985) Semliki Forest virus infection of mice: a model for genetic and molecular analysis of viral pathogenicity. J Gen Virol 66: 395–408
    Google Scholar
  3. Barrett ADT, Dimmock NJ (1984) Variation in homotypic and heterotypic interference by defective interfering viruses derived from different strains of Semliki Forest virus and from Sindbis virus. J Gen Virol 65: 1119–1122
    Google Scholar
  4. Bradish CJ, Allner K (1972) The early responses of mice to respiratory or intra-peritoneal infection by defined strains of Semliki Forest virus. J Gen Virol 25: 205–218
    Google Scholar
  5. Bradish CJ, Allner K, Maber HB (1971) The virulence of original and derived strains of Semliki Forest virus for mice, guinea-pigs, and rabbits. J Gen Virol 12: 141–162
    Google Scholar
  6. Clarke DH, Casals J (1958) Techniques for hemagglutination and hemagglutination-inhibition with arthropode-borne viruses. Am J Trop Med Hyg 7: 561–573
    Google Scholar
  7. Crouch CF, Mackenzie A, Dimmock NJ (1982) The effect of defective-interfering Semliki Forest virus on the histopathology of infection with virulent Semliki Forest virus in mice. J Inf Dis 146: 411–416
    Google Scholar
  8. Dimmock NJ, Kennedy SIT (1978) Prevention of death in Semliki Forest virus-infected mice by administration of defective-interfering Semliki Forest virus. J Gen Virol 39: 231–242
    Google Scholar
  9. Esiri MM (1984) Herpes simplex encephalitis. Immunohistological demonstration of spread of virus via olfactory and trigeminal pathways after infection of facial skin in mice. J Neurol Sci 64: 213–217
    Google Scholar
  10. Fleming P (1977) Age-dependent and strain-related differences of virulence of Semliki Forest virus in mice. J Gen Virol 37: 93–105
    Google Scholar
  11. Garoff H, Frischauf A-M, Simons K, Lehrbach H, Delius H (1980) Nucleotide sequence of cDNA coding for Semliki Forest virus membrane glycoproteins. Nature 288: 236–241
    Google Scholar
  12. Gates MC, Sheahan BJ, Atkins GJ (1984) The pathogenicity of the M 9 mutant of Semliki Forest virus in immunocompromised mice. J Gen Virol 65: 73–80
    Google Scholar
  13. Graham RL, Karnowsky MJ (1966) The early stages of absorption of injected horse-raddish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem Immunol 14: 291–302
    Google Scholar
  14. Henderson DW, Peacock S, Randles WJ (1967) On the pathogenesis of Semliki Forest virus (SFV) infection in the hamster. Br J Exp Pathol 48: 228–234
    Google Scholar
  15. Johnson RT, Mims A (1968) Pathogenesis of viral infections of the nervous system. N Engl J Med 278: 23–30, 84–92
    Google Scholar
  16. Kaluza G (1976) Early synthesis of Semliki Forest virus-specific proteins in infected chicken cells. J Virol 19: 1–12
    Google Scholar
  17. MacFarlan RI, Burns WH, White DO (1977) Two cytotoxic cells in peritoneal cavity of virus-infected mice: antibody-dependent macrophages and nonspecific killer cells. J Immunol 119: 169–574
    Google Scholar
  18. Matsumoto S (1970) Rabies virus. Adv Virus Res 16: 257–301
    Google Scholar
  19. Mussgay M, Weiland M, Strohmeier K, Überschär S, Enzmann PJ (1973) Properties of components obtained by treatment of Semliki Forest virus with Tween 80 and tri(n-butyl)-phosphate. J Gen Virol 19: 89–101
    Google Scholar
  20. Nir Y, Beemer A, Goldwasser RA (1965) West Nile virus infection in mice following exposure to a viral aerosol. Br J Exp Pathol 46: 443–449
    Google Scholar
  21. Pathak S, Webb HE (1974) Possible mechanisms for the transport of Semliki Forest virus into and within mouse brain. An electron-microscopic study. J Neurol Sci 23: 175–184
    Google Scholar
  22. Pusztai R, Gould EA, Smith H (1971) Infection patterns in mice of an avirulent and virulent strain of Semliki Forest virus. Br J Exp Pathol 52: 669–677
    Google Scholar
  23. Reinacher M, Bonin J, Narayan O, Scholtissek C (1983) Pathogenesis of neurovirulent Influenza A virus infection in mice: route of entry of virus into brain determines infection of different populations of cells. Lab Invest 49: 686–692
    Google Scholar
  24. Rott R, Reinacher M, Orlich M, Klenk H-D (1980) Cleavability of hemagglutinin determines spread of avian influenza viruses in the chorioallantoic membrane of chicken embryo. Arch Virol 65: 123–133
    Google Scholar
  25. Smithburn KC, Haddow AJ (1944) Semliki Forest virus. I. Isolation and pathogenic properties. J Immunol 49: 141–157
    Google Scholar
  26. Stefanini M, de Martino C, Zamboni L (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature 216: 173–174
    Google Scholar
  27. Streefkerk JG (1972) Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol. J Histochem Cytochem 20: 829–831
    Google Scholar
  28. Wagner RR, Levy AH, Smith TJ (1968) Techniques for the studies of interferons in animal virus-cell systems. In:Maramorosh K, Koprowski H (eds) Methods in virology, vol IV. Academic Press, New York, pp 1–52
    Google Scholar
  29. Willems WR, Kaluza G, Bauer H, Hager H, Schuetz H-J, Feistner H (1979) Semliki Forest Virus: cause of a fatal case of human encephalitis. Science 203: 1127–1129
    Google Scholar
  30. Zimmermann T, Schäfer W (1960) Effect of p-fluorophenylalanine on fowl plague virus multiplication. Virology 11: 676–698
    Google Scholar

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Authors and Affiliations

  1. Institut für Virologie, Justus-Liebig-Universität Giessen, Giessen, Germany
    G. Kaluza, G. Lell, L. Stitz & W. R. Willems
  2. Institut für Veterinär-Pathologie, Justus-Liebig-Universität Giessen, Giessen, Germany
    M. Reinacher

Authors

  1. G. Kaluza
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  2. G. Lell
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  3. M. Reinacher
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  4. L. Stitz
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  5. W. R. Willems
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This work was supported by the Sonderforschungsbereich 47 (Pathogenitätsmechanismen von Viren).

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Kaluza, G., Lell, G., Reinacher, M. et al. Neurogenic spread of Semliki forest virus in mice.Archives of Virology 93, 97–110 (1987). https://doi.org/10.1007/BF01313896

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