Identification of two complementation groups in Fanconi anemia (original) (raw)

Abstract

Considerable variation can be observed in the clinical presentation of Fanconi anemia (FA) patients and in the degree of sensitivity of their cells to DNA damaging agents. We have examined the hypothesis that genetic heterogeneity underlies this variation by testing for complementation in somatic cell hybrids constructed from FA cells. Hybrids were formed by fusing lymphoblastoid cell lines derived from four different FA patients. Complementation of the cellular defects in FA was tested by examining sensitivity to growth inhibition by mitomycin C(MMC), spontaneous chromosome breakage, and MMC-induced chromosome breakage in the hybrid cells. These studies revealed the presence of at least two complementation groups, suggesting that there may be two or more different FA genes.

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Literature cited

  1. Schroeder, T.M., Tilgen, D., Kruger, J., and Vogel, F. (1976).Hum. Genet. 32:257–288.
    PubMed Google Scholar
  2. Swift, M. (1971).Nature 230:370–373.
    PubMed Google Scholar
  3. Schroeder, T.M., Anschutz, F., and Knopp, A. (1964).Humangenetik 1:194–196.
    PubMed Google Scholar
  4. Sasaki, M.S., and Tonomura, A. (1973).Cancer Res. 33:1829–1836.
    PubMed Google Scholar
  5. Glanz, A., and Clarke-Fraser, F. (1982).J. Med. Genet. 19:412–416.
    PubMed Google Scholar
  6. Duckworth-Rysiecki, G., Hulten, M., Mann, J., and Taylor, A.M.R. (1984).J. Med. Genet. 21:197–203.
    PubMed Google Scholar
  7. Higurashi, M., and Conen, P.E. (1973).Cancer 32:380–383.
    PubMed Google Scholar
  8. Arlett, C.F., and Harcourt, S.A. (1980).Cancer Res. 40:926–932.
    PubMed Google Scholar
  9. Weichselbaum, R.R., Nove, J., and Little, J.B. (1980).Cancer Res. 40:920–925.
    PubMed Google Scholar
  10. Ishida, R., and Buchwald, M. (1982).Cancer Res. 42:4000–4006.
    PubMed Google Scholar
  11. Weksberg, R., Buchwald, M., Sargent, P., Thompson, M.W., and Siminovitch, L. (1979).J. Cell. Physiol. 101:311–324.
    PubMed Google Scholar
  12. De Weerd-Kastelein, E.A., Keijzer, R., and Bootsma, D. (1972).Nature (London), New Biol. 238:80–83.
    Google Scholar
  13. Jaspers, N.G.J., and Bootsma, D. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:2641–2644.
    PubMed Google Scholar
  14. Baker, R.M., Brunette, D.M., Mankowitz, R., Thompson, L.M., Whitmore, G.F., Siminovitch, L., and Till, J.E. (1974).Cell 1:9–21.
    Google Scholar
  15. Nordenson, I., Bjorksten, B., and Lundh, B. (1980).56:169–171.
  16. Yoshida, M.C. (1980).Hum. Genet. 55:223–226.
    PubMed Google Scholar
  17. Yoshida, M.C. (1982).Cytogenet. Cell Genet. 32:329–330.
    Google Scholar
  18. Zakrzewski, S., and Sperling, K. (1980).Hum. Genet. 56:81–84.
    PubMed Google Scholar
  19. Zakrzewski, S., and Sperling, K. (1983).Hum. Genet. 62:321–323.
    Google Scholar
  20. Zakrzewski, S., Koch, M., and Sperling, K. (1983).Hum. Genet. 64:55–57.
    PubMed Google Scholar

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Authors and Affiliations

  1. Research Institute, The Hospital for Sick Children, Toronto, Ontario
    G. Duckworth-Rysiecki, K. Cornish, C. A. Clarke & M. Buchwald
  2. Departments of Medical Genetics and Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
    G. Duckworth-Rysiecki, K. Cornish, C. A. Clarke & M. Buchwald

Authors

  1. G. Duckworth-Rysiecki
  2. K. Cornish
  3. C. A. Clarke
  4. M. Buchwald

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Duckworth-Rysiecki, G., Cornish, K., Clarke, C.A. et al. Identification of two complementation groups in Fanconi anemia.Somat Cell Mol Genet 11, 35–41 (1985). https://doi.org/10.1007/BF01534732

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