Fast-twitch and slow-twitch/cardiac Ca2+ ATPase genes map to human chromosomes 16 and 12 (original) (raw)

Abstract

The fast-twitch and slow-twitch/cardiac Ca 2+ ATPase genes have been assigned to human chromosomes 16 and 12, respectively, using rodent-human somatic cell hybrids and filter hybridization analysis of cell hybrid DNA. A rabbit cDNA for the fast-twitch ATPase hybridizes to a prominent single fragment in human genomic DNA digested with the restriction enzyme BamHI. By correlating the presence of this fragment in somatic cell hybrid DNA with the human chromosome content of the hybrids, the fast-twitch ATPase gene can be assigned to human chromosome 16. A slow-twitch/cardiac ATPase cDNA clone was isolated from a human muscle cDNA library and used to detect human fragments in EcoRI-digested somatic cell hybrid DNA. By correlating the presence of these fragments with the human chromosome content of the hybrids, the slow-twitch/cardiac ATPase gene can be assigned to human chromosome 12. Thus, the two ATPase genes, which are probably related to each other by an ancient duplication event, are not syntenic in the human genome.

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Authors and Affiliations

  1. Banting and Best Department of Medical Research, University of Toronto, C.H. Best Institute, 112 College Street, M5G 1L6, Toronto, Ontario, Canada
    David H. MacLennan & Christopher J. Brandl
  2. Montreal Neurological Institute, 3801 University Street, H3A 2B4, Montreal, Quebec, Canada
    Shashikant Champaneria & Paul C. Holland
  3. Department of Medical Genetics, University of Toronto, M5S 1A8, Toronto, Ontario, Canada
    Vicki E. Powers & Huntington F. Willard

Authors

  1. David H. MacLennan
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  2. Christopher J. Brandl
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  3. Shashikant Champaneria
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  4. Paul C. Holland
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  5. Vicki E. Powers
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  6. Huntington F. Willard
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MacLennan, D.H., Brandl, C.J., Champaneria, S. et al. Fast-twitch and slow-twitch/cardiac Ca2+ ATPase genes map to human chromosomes 16 and 12.Somat Cell Mol Genet 13, 341–346 (1987). https://doi.org/10.1007/BF01534928

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