Agrocin-producing pathogenic and nonpathogenic biotype-3 strains ofAgrobacterium tumefaciens active against biotype-3 pathogens (original) (raw)

Abstract

Agrocin-producing pathogenic and nonpathogenic biotype-3 strains of_Agrobacterium tumefaciens_ were isolated from grapevine gall tissue. In vitro activity of the nonpathogenic agrocin producers was restricted to biotype-3 pathogens used. Pathogenic agrocin producers were active in vitro against biotype-3 agrocin-producing nonpathogens, non-agrocin-producing pathogens, and biotype-1 strains when cultivated on a modified Stonier's medium; on a medium designated AB, two strains stested showed no activity against agrocin-producing nonpathogens, but agrocin of one of these strains was active against other agrocin-producing pathogens. In a greenhouse experiment a marked tendency toward decreased gall formation by biotype-3 pathogens on grapevines was obtained when biotype-3 pathogens and nonpathogenic biotype-3 agrocin producers were applied to wounds simultaneously. In this experiment, agrocin-producting pathogens tended to be more virulent than non-agrocin-producing pathogens.

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Literature Cited

  1. Allen ON (1959) Experiments in soil bacteriology. Minneapolis: Burgess
    Google Scholar
  2. Bernaerts MJ, De Ley J (1963) A biochemical test for crown gall bacteria. Nature (Lond) 197:406–907
    Google Scholar
  3. Brisbane PG, Kerr A (1983) Selective media for the three biovars of_Agrobacterium_. J. Appl Bacteriol 54:425–431
    Google Scholar
  4. Clarck AG (1969) A selective medium for the isolation of_Agrobacterium_ species. J Appl Bacteriol 32:348–351
    Google Scholar
  5. Cooksey DA, Moore LW (1980) Biological control of crown gall with fungal and bacterial antagonists. Phytopathology 70:506–509
    Google Scholar
  6. Cooksey DA, Moore LW (1982) Biological control of crown gall with an agrocin mutant of_Agrobacterium radiobacter_. Phytopathology 72:919–921
    Google Scholar
  7. Ellis JG, Kerr A, Van Montagu M, Schell J (1979)Agrobacterium: genetic studies on agrocin 84 production and the biological control of crown gall. Physiol Plant Pathol 15:311–319.
    Google Scholar
  8. Engler G, Holster M Van Montagu M, Schell J, Hernalsteens JP, Schilperoort R (1975) Agrocin 84 sensitivity: a plasmid determined property of_Agrobacterium tumefaciens_. Mol Gen Genet 138:345–349
    PubMed Google Scholar
  9. Hendson M, Askjaer L, Thomson JA, Van Montagu M (1983) Broad-host-range agrocin of_Agrobacterium tumefaciens_. Appl Environ Microbiol 45:1526–1532
    Google Scholar
  10. Htay K, Kerr A (1974) Biological control of crown gall: seed and root inoculation. J Appl Bacteriol 37:525–530
    PubMed Google Scholar
  11. Kechru RB, Singh RN, Yadav IS (1972) Physiological studies on dormancy in grape seeds (Vitis vinifera var. Black Muscat). II. On the effect of growth substances, low chilling temperature and subjection of the seeds to running water. Vitis 11:289–295
    Google Scholar
  12. Kerr A (1972) Biological control of crown gall: seed inoculation. J Appl Bacteriol 35:493–497
    Google Scholar
  13. Kerr A (1974) Soil microbiological studies on_Agrobacterium radiobacter_ and biological control of crown gall. Soil Sci 118:168–172
    Google Scholar
  14. Kerr A (1980) Biological control of crown gall through production of agrocin 84. Plant Dis 64:25–40
    Google Scholar
  15. Kerr A, Htay K (1974) Biological control of crown gall through bacteriocin production. Physiol Plant Pathol 4:37–44
    Google Scholar
  16. Kerr A, Panagopoulos CG (1977) Biotypes of_Agrobacterium radiobacter_ var.tumefaciens and their biological control. Phytopathol Z 90:172–179
    Google Scholar
  17. Kerr A, Roberts WP (1976)Agrobacterium: correaltions between transfer of pathogenicity, octopine and nopaline metabolism and bacteriocin 84 sensitivity. Physiol Plant Pathol 9:205–211
    Google Scholar
  18. Lippincott BB, Lippincott JA (1969) Bacterial attachment to a specific wound site as an essential stage in tumor initiation by_Agrobacterium tumefaciens_. J Bacteriol 97:620–628
    PubMed Google Scholar
  19. Loubser JT (1978) Identification of_Agrobacterium tumefaciens_ biotype 3 on grapevine in South Africa. Plant Dis Rep 62:730–731
    Google Scholar
  20. McCardell BA, Pootjes CF (1976) Chemical nature of agrocin 84 and its effect on a virulent strain of_Agrobacterium tumefaciens_. Antimicrob Agents Chemother 10:498–502
    PubMed Google Scholar
  21. Moore LW (1977) Prevention of crown gall on_Prunus_ roots by bacterial antagonists. Phytopathology 67:139–144
    Google Scholar
  22. Moore LW, Warren G (1979)Agrobacterium radiobacter strain 84 and biological control of crown gall. Annu Rev Phytopathol 17:163–179
    Google Scholar
  23. New PB, Kerr A (1971) A selective medium for_Agrobacterium radiobacter_ biotype 2. J Appl Bacteriol 34:233–236
    PubMed Google Scholar
  24. New PB, Kerr A (1972) Biological control of crown gall: field measurements and glasshouse experiments. J Appl Bacteriol 35:279–287
    Google Scholar
  25. Panagopoulos CG, Psallidas PG, Alivizatos AS (1978) Studies on biotype 3 of_Agrobacterium radiobacter_ var.tumefaciens. In: Proceedings of the fourth international conference on pathological bacteria. Angers, France: Gilbert, pp 221–228
    Google Scholar
  26. Schroth MN, Thompson JR, Hildebrandt DC (1965) Isolation of_Agrobacterium tumefaciens—A. radiobacter_ group from soil. Phytopathology 55:645–647
    Google Scholar
  27. Stonier T (1960)Agrobacterium tumefaciens Conn. II. Production of an antibiotic substance. J Bacteriol 79:889–898
    PubMed Google Scholar
  28. Vincent JM (1970) A manual for the practical study of rootnodule bacteria, 1st edn. IBP handbook 15, Oxford: Blackwell
    Google Scholar

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Authors and Affiliations

  1. Plant Protection Research Institute, Private Bag X134, 0001, Pretoria, South Africa
    Johannes L. Staphorst, Ferdinand G. H. van Zyl, Barend W. Strijdom & Zandra E. Groenewold

Authors

  1. Johannes L. Staphorst
  2. Ferdinand G. H. van Zyl
  3. Barend W. Strijdom
  4. Zandra E. Groenewold

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Staphorst, J.L., van Zyl, F.G.H., Strijdom, B.W. et al. Agrocin-producing pathogenic and nonpathogenic biotype-3 strains of_Agrobacterium tumefaciens_ active against biotype-3 pathogens.Current Microbiology 12, 45–52 (1985). https://doi.org/10.1007/BF01567753

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