Identification of the theta-type minimal replicon of theLactococcus lactis subsp.lactis CNRZ270 lactose protease plasmid pUCL22 (original) (raw)

Abstract

The replication region of pUCL22, the lactose-protease plasmid of_Lactococcus lactis_ subsp.lactis (Lc. lactis) CNRZ270, was isolated on an 18-kb_Bam_HI fragment, by cloning into pUCB300, an_Escherichia coli_ vector encoding Emr, and selecting for Emr in_Lc. lactis_ MG1614. Subcloning and deletion analysis localized the replication region, named_Rep_22, on a 2.3-kb fragment. Replicons based on this region followed a theta-type mechanism of replication in_Lc. lactis_. An internal 1251-bp_Dra_I fragment of_Rep_22 used as a probe hybridized with numerous plasmids in bacteria from the genera_Lactococcus, Lactobacillus_, and_Leuconostoc_. In some strains, two or three coresident plasmids hybridized with the probe in stringent conditions. It appears, therefore, that this family of theta-type replicons is widely distributed in lactic acid bacteria and contains several incompatibility groups.

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Authors and Affiliations

  1. Laboratory of Microbial Genetics, IRBA, University of Caen, 14032, Caen, France
    Jacques Frère, Abdellah Benachour, Madeleine Novel & Georges Novel

Authors

  1. Jacques Frère
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  2. Abdellah Benachour
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  3. Madeleine Novel
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  4. Georges Novel
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Frère, J., Benachour, A., Novel, M. et al. Identification of the theta-type minimal replicon of the_Lactococcus lactis_ subsp.lactis CNRZ270 lactose protease plasmid pUCL22.Current Microbiology 27, 97–102 (1993). https://doi.org/10.1007/BF01570865

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