The Bacillus megaterium ribonucleotide reductase: Evidence for a B12 coenzyme requirement (original) (raw)

Summary

On the basis of the following evidence, it has been concluded that extracts of_Bacillus megaterium_ KM contain a B12 coenzyme-dependent ribonucleotide reductase. The reduction of cytidine nucleotides to deoxycytidine nucleotides is enhanced by the addition of B12 coenzyme. In addition, the presence of hydroxyurea, a specific inhibitor of non-B12 coenzymedependent reductases, has only a slight effect on this reduction. Finally,B. megaterium extracts catalyze a transfer of tritium from 5′-deoxyadenosylcobalamin-5′-3H2 to water, a reaction specific for B12 coenzyme-dependent ribonucleotide reductases.

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Authors and Affiliations

  1. Depart. of Microbiol., University of Illinois, 61801, Urbana, Illinois, USA
    S. Yau & J. T. Wachsman

Authors

  1. S. Yau
  2. J. T. Wachsman

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Yau, S., Wachsman, J.T. The Bacillus megaterium ribonucleotide reductase: Evidence for a B12 coenzyme requirement.Mol Cell Biochem 1, 101–105 (1973). https://doi.org/10.1007/BF01659943

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