Oligonucleotide formation catalyzed by mononucleotide matrices (original) (raw)
Summary
Pb2+-containing precipitates of mononucleotides form matrices which catalyze the self-condensation of nucleotide 5′-phosphorimidazolides and their condensation with nucleosides. The reactions exhibit base-pairing specificity between matrix nucleotide and substrate, and usually follow the Watson-Crick pairing rules. Although purine polynucleotides do not facilitate the oligomerization of pyrimidine nucleotide monomers in solution, it is interesting that purine-containing matrices do catalyze such a reaction. The significance of the results in the context of the prebiotic evolution of polynucleotides is discussed.
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Abbreviations
Im:
imidazole
EDTA:
ethylenediaminetetraacetic acid
A:
adenosine
C:
cytidine
CMe:
5-methylcytidine
Cara:
9-_β_-D-arabinofuranosylcytosine
G:
guanosine
I:
inosine
U:
uridine
Uara:
9-_β_-D-arabinofuranosyluracil
dA:
2′-deoxyadenosine
dC:
2′-deoxycytidine
dG:
2′-deoxyguanosine
T:
thymidine
pN (N = A, C, G, I, U, Uara):
ribonucleoside 5′-phosphate
pdN (dN = dA, dC, dG, T):
deoxynucleoside 5′-phosphate
Np:
ribonucleoside 2′(3′)-phosphate
N>p:
nucleoside cyclic 2′,3′-phosphate
ImpN:
5′-phosphorimidazolide of a nucleoside N
NppN:
P1,P2-dinucleoside 5′-diphosphate
pNp:
5′-phosphonucleoside 2′(3′)-phosphate
N(pN)n (n = 1, 2⋯):
oligonucleotide terminated by a free 5′-OH group
(pN)n (n = 2. 3⋯):
oligomers of pN
(Np)n (n = 2, 3⋯):
oligomers of Np
p2N:
nucleoside 5′-diphosphate
p3N:
nucleoside 5′-triphosphate
poly(N):
polynucleotide derived from N. (Pb-pN) or (Np-Pb), matrix formed by precipitation of a nucleoside pN or Np, respectively, with Pb2+ ions
e.g.\(\mathop U\limits^* \) 3pU:
[2-14 C]-uridylyl-[3′ → 5′]-uridine
OD:
optical density units measured at 259 nm
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Authors and Affiliations
- The Salk Institute for Biological Studies, 92138, San Diego, California, USA
R. Lohrmann
Additional information
The numbers given as superscripts between the symbols of a nucleoside and a phosphate indicate the type of internucleotide linkage e.g. A2pA is adenylyl-[2′ → 5′]-adenosine; a star above the symbol for a nucleoside indicates the position of a radioactive label
The numbers given as superscripts between the symbols of a nucleoside and a phosphate indicate the type of internucleotide linkage e.g. A2pA is adenylyl-[2′ → 5′]-adenosine; a star above the symbol for a nucleoside indicates the position of a radioactive label
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Lohrmann, R. Oligonucleotide formation catalyzed by mononucleotide matrices.J Mol Evol 18, 185–195 (1982). https://doi.org/10.1007/BF01733045
- Received: 05 March 1981
- Accepted: 19 June 1981
- Issue date: May 1982
- DOI: https://doi.org/10.1007/BF01733045