The use of ribonuclease U2 in RNA sequence determination (original) (raw)

Abstract

The catalog of oligomers produced by ribonuclease T1 digestion of_Escherichi coli_ 16S ribosomal RNA has been determined by a new method that involves the use of ribonuclease U2 from_Ustilago sphaerogena._ The sequences for the larger T1 oligomers (8 or more bases) determined in this way differ in more than 50 % of the cases from those reported previously (determined by other methods).

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Author notes

1. T. Uchida
   Present address: Mitsubishi-Kasei Institute of Life Sciences, Machida, Tokyo, Japan

Authors and Affiliations

1. Department of Microbiology, University of Illinois, 61801, Urbana, Illinois, USA
   T. Uchida, L. Bonen, H. W. Schaup, B. J. Lewis, L. Zablen & C. Woese

Authors

1. T. Uchida
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2. L. Bonen
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3. H. W. Schaup
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4. B. J. Lewis
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5. L. Zablen
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6. C. Woese
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Uchida, T., Bonen, L., Schaup, H.W. et al. The use of ribonuclease U2 in RNA sequence determination.J Mol Evol 3, 63–77 (1974). https://doi.org/10.1007/BF01795977

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• Received: 23 June 1973
• Issue Date: March 1974
• DOI: https://doi.org/10.1007/BF01795977

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