Western blot analysis of antibody responses of young infants to pertussis infection (original) (raw)

Abstract

Western blot and agglutination techniques were used to analyze the antibody responses to_Bordetella pertussis_ in 27 infants less than six month of age with presumed pertussis infection. The antibody response to the_Bordetella pertussis_ adhesins filamentous hemagglutinin, pertactin and agglutinogens, and to the_Bordetella pertussis_ toxins pertussis toxin and adenylate cyclase-hemolysin were compared. Infection induced intense antibody responses to filamentous hemagglutinin, pertussis toxin and adenylate cyclase-hemolysin. Antibodies to agglutinogens were never detected, and antibodies to pertactin were rarely detected in infected infants' sera. Therefore, determination of anti-agglutinogens levels only is not suitable for the serological diagnosis of pertussis in young infants. Use of purified filamentous hemagglutinin, pertussis toxin and adenylate cyclase-hemolysin in Western blot analysis may improve the serodiagnosis of_Bordetella_ infections. However, care must be exercised in distinguishing between the antibody response in young infants and maternally derived antibodies.

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Authors and Affiliations

  1. Institut Pasteur, Unité de Bactériologie Moléculaire et Médicale, 28 Rue du Dr. Roux, 75724, Paris Cedex 15, France
    N. Guiso & E. Grimprel
  2. Consultation de Pédiatrie, Pathologie Infectieuse et Tropicale, Urgences Pédiatriques, Hôpital d'Enfants Armand Trousseau, 26 Avenue du Dr. Arnold Netter, 75571, Paris Cedex 12, France
    E. Grimprel, I. Anjak & P. Bégué

Authors

  1. N. Guiso
  2. E. Grimprel
  3. I. Anjak
  4. P. Bégué

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Guiso, N., Grimprel, E., Anjak, I. et al. Western blot analysis of antibody responses of young infants to pertussis infection.Eur. J. Clin. Microbiol. Infect. Dis. 12, 596–600 (1993). https://doi.org/10.1007/BF01973637

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