In vitro activation of hepatic glutathione reductase from mice by lobenzarit disodium (original) (raw)
Abstract
Glutathione reductase activity from mice liver is significantly enhanced by lobenzarit disodium at concentrations between 0.3 and 1.5 m_M_. A maximum activation of almost 30% is achieved at a drug concentration of 0.9 m_M_. Similar results were observed with glutathione reductase from human leukocytes, but not with the enzyme from yeast. By preincubation with the enzyme from mice liver, lobenzarit also proved to prevent, at least partially, the immediate inhibition caused by the well-known thiol-reacting agents, thus indicating a protecting effect on the catalytically important thiol residue of the enzyme. The results here obtained explain in part the recently found hepatoprotective effect of lobenzarit disodium against acute liver toxicity induced by acetaminophen in mice.
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References
- O. Cynshi, M. Saitoh, F. Cynshi, M. Tanemura, S. Hata and M. Nakano,Antioxidative profile of lobenzarit disodium (CCA). Biochem. Pharmacol.40, 2117–2122 (1990).
Google Scholar - Y. Shiokawa, Y. Horiuchi, Y. Mizushima, T. Kageyama, K. Schichikawa, T. Ohfuji, M. Honma, H. Yoshizawa, C. Abe and N. Ogawa,A multicenter double-blind control study of lobenzarit, a novel immunomodulator in rheumatoid arthritis. J. Rheumatol.11, 615–623 (1984).
Google Scholar - R. Gonzalez, C. Pascual, O. Ancheta, B. Carreras, D. Remirez and R. Pellon,Hepatoprotective effects of lobenzarit disodium on acetaminophen-induced liver damage in mice. Agents and Actions_37_, 114–120 (1992).
Google Scholar - R. Pellon, V. Millian and R. Carrasco,Procedimiento para la preparacion de acidos N-fenilantranilicos sustituidos. Cuban Patent No. 22105, 1992.
- Y. Suzuki, M. Kikuchi, T. Morita, M. Haneda, H. Nagai, M. Itoh, Y. Yutani, Y. Kuriki, K. Mituhashi and M. Shiba,Physicochemical properties and stability of lobenzarit disodium. Iyakuhin Kenkyu.15 (2), (1984).
- C. E. Mize and R. G. Langdon,_He[atic glutathione reductase. Purification and general kinetic properties_. J. Biol. Chem._237_, 1589–1595 (1962).
Google Scholar - H. Galjaard, Genetic Metabolic Diseases. Early Diagnosis and Prenatal Analysis, p. 791. Elsevier, North-Holland, Biomedical Press Amsterdam, New York 1980.
Google Scholar - I. K. Smith, T. L. Vierheller and C. A. Thorne,Assay of glutathione reductase in crude tissue homogenates using 5,5′-ditiobis (2-nitrobenzoic acid). Anal Biochem.175, 408–413 (1988).
Google Scholar - O. H. Lowry, N. H. Rosenbrough, A. L. Farr and R. J. Randall,Protein measurement with the folin phenol reagent. J. Biol. Chem.193, 265–275 (1951).
Google Scholar
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Authors and Affiliations
- Department of Clinical Biochemistry, National Center for Scientific Research, 6990, Havana, Cuba
Julio Armesto, Nancy Frutos, Ricardo Gonzalez & Carlos Pascual
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- Julio Armesto
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Armesto, J., Frutos, N., Gonzalez, R. et al. In vitro activation of hepatic glutathione reductase from mice by lobenzarit disodium.Agents and Actions 39, 69–71 (1993). https://doi.org/10.1007/BF01975716
- Received: 15 September 1992
- Accepted: 21 January 1993
- Issue Date: May 1993
- DOI: https://doi.org/10.1007/BF01975716