Culture ofBorrelia burgdorferi on six solid media (original) (raw)

Abstract

Six solid substrates were compared for their support of growth of ten isolates of_Borrelia burgdorferi_, the causative agent of Lyme borreliosis. The following substrates with or without rabbit serum and bovine serum albumin were tested: BSK agar, BHIAM agar, TAROM agar, MEM agar, MKP agar and PMR agar. After incubation in a candle jar and a GasPak for two to four weeks,Borrelia colonies were counted and characterized. Colony morphology was related more to the growth substrate than to the characteristics of the various_Borrelia burgdorferi_ isolates. Culture on PMR agar resulted in the highest recovery rate and the best colony formation, with a size variation of 0.3–3.0 mm. Culture of_Borrelia burgdorferi_ on solid media may facilitate the diagnosis of Lyme borreliosis by enabling easier biological and genetical analysis of isolates and more accurate differentiation of strains.

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Authors and Affiliations

  1. Max von Pettenkofer Institut, Ludwig-Maximillian-Universität, Pettenkoferstr. 9a, 8000, Munich 2, Germany
    V. Preac-Mursic, B. Wilske & S. Reinhardt

Authors

  1. V. Preac-Mursic
  2. B. Wilske
  3. S. Reinhardt

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Preac-Mursic, V., Wilske, B. & Reinhardt, S. Culture of_Borrelia burgdorferi_ on six solid media.Eur. J. Clin. Microbiol. Infect. Dis. 10, 1076–1079 (1991). https://doi.org/10.1007/BF01984935

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