Zonal analysis of phospholipids in the epiphyseal cartilage and bone of normal and rachitic chickens and pigs (original) (raw)
Abstract
The effect of various degrees of simple, vitamin D-deficient rickets on the levels, composition and extractability of phospholipids from calcifying and soft tissues was studied in chickens and pigs. A variety of effects of rickets on the phospholipid composition of the calcifying tissues were detected; there was little change in the soft tissues. The quantitative effects on the lipid concentration of rachitic hard tissues were variable from zone to zone and appeared to reflect variations in other tissue constituents. However, the qualitative differences indicated a direct effect on specific phospholipids. Acidic phospholipids displayed the greatest percentage change, although numerous statistically-significant changes were also seen in the neutral phospholipids. Tissue zones displaying the greatest change in phospholipid composition in rickets were proliferating and hypertrophic cartilage, and cancellous bone, with calcified cartilage showing the least effect. Rickets also reduced the extractability of certain acidic and neutral phospholipids from zones of early mineralization. These findings are interpreted as indicating the presence of elevated levels of phospholipid complexed with calcium phosphate in rachitic tissue, presumably due to the blockage of normal mineralization.
Résumé
Les effets de divers degrés de rachitisme non compliqué, provoqué par avitaminose D, sur les concentrations, la composition, et l'extractibilité de phospholipides de tissus moux et en voie de calcification ont été étudiés chez le poulet et le porc. Le contenu en phospholipides des tissus en voie de calcification est modifié au cours du rachitisme; cependant, on n'a constaté que peu de changements dans les tissus moux. Les effets quantitatifs sur la concentration en lipides des tissus rachitiques calcifiés varient de zone en zone et semblent indiquer desvariations dans d'autres constituants tissulaires. Cependant les différences qualitatives indiquent un effet direct sur des phospholipides spécifiques. Les phospholipides acides présentent les changements de pourcentage les plus élevés bien que de nombreuses modifications statisstiquement significatives dans les phospholipides neutres ont pu être observés. Les zones stisulaires présentant le plus grand changement de composition phospholipidique au cours du rachitisme, sont constituées par le cartilage hypertrophique et en voie de prolifération et par l'os spongieux, alors que le cartilage calcifié est le moins affecté. Le rachitisme diminue aussi l'extractibilité de certains phospholipides neutres et acides des zones de minéralisation précoce. Ces résultats semblent indiquer la présence, dans le tissu rachitique, de concentrations élevées de phospholipide complexé avec le phosphate de calcium, probablement causé par l'inhibition de la minéralisation normale.
Zusammenfassung
Die Auswirkung von verschiedenen Graden von Rachitis (verursacht durch Vitamin D-Mangel) auf die Mengen, die Zusammensetzung und auf die Extrahierbarkeit der Phospholipide von verkalkenden und weichen Geweben wurde an Hühnern und Schweinen untersucht. Es wurden verschiedene Wirkungen von Rachitis auf die Phospholipid-Zusammensetzung der verkalkenden Gewebe gefunden, in den weichen Geweben jedoch nur wenige. Der Grad dieser Wirkung auf die Lipid-Konzentration der rachitisch verknöchernden Gewebe änderte sich von Schicht zu Schicht und schien die Änderungen in anderen Gewebebestandteilen wiederzugeben. Die qualitativen Unterschiede zeigten jedoch eine direkte Wirkung auf spezifische Phospholipide an. Saure Phospholipide zeigten den größten prozentualen Unterschied, obgleich zahlreiche statistisch signifikante Unterschiede auch in den neutralen Phospholipiden gefunden wurden. Die Gewebeschichten, welche bei Rachitis die größte Änderung in der Phospholipidzusammensetzung zeigten, waren proliferierender und hypertrophischer Knorpel und spongiöser Knochen, während verkalkter Knorpel die geringste Änderung zeigte. Rachitis verminderte auch die Extrahierbarkeit von gewissen sauren und neutralen Phospholipiden aus den Schichten der frühen Mineralisierung. Diese Ergebnisse weisen auf das Vorhandensein von erhöhten Phospholipidmengen im rachitischen Gewebe hin, welche Calciumphosphat binden; dies wird wahrscheinlich durch die Blockierung der normalen Mineralisierung verursacht.
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Rickets
Article 21 December 2017
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Authors and Affiliations
- Department of Biochemistry, University of Vermont College of Medicine, 05401, Burlington, Vermont, USA
R. E. Wuthier - Department of Orthopaedic Surgery, University of vermont College of Medicine, 05401, Burlington, Vermont, USA
R. E. Wuthier
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Wuthier, R.E. Zonal analysis of phospholipids in the epiphyseal cartilage and bone of normal and rachitic chickens and pigs.Calc. Tis Res. 8, 36–53 (1971). https://doi.org/10.1007/BF02010121
- Received: 24 August 1970
- Accepted: 21 December 1970
- Issue date: December 1971
- DOI: https://doi.org/10.1007/BF02010121