Detection of nine chitinase species in germinating cells ofMucor rouxii (original) (raw)
Abstract
Chitinolytic activity in aerobically germinating cells of_Mucor rouxii_ was studied. When an ammonium sulfate fraction (40% saturation) of cytosol was subjected to column chromatography in Bio-Gel P-100, two peaks of activity were separated: A and B, which showed molecular weights of 53 KDa and 28 KDa, respectively. After further purification by ion exchange chromatography in a column of DEAE Bio-Gel A, the lytic activity from peaks A and B resolved into four and five species, respectively. Species from peak A eluted with peaks at 0.075, 0.125, 0.160, and 0.225_M_ NaCl, whereas those from peak B did so at 0.050, 0.125, 0.190, 0.240. and 0.300_M_ NaCl. Like other chitinases from the same fungus, the ones detected here are of the exochitinolytic type, releasing diacetylchitobiose units from nascent chitin chains. The complex chitinolytic system in these cells is discussed and compared with that exhibited by exponentially growing cells of the same fungus.
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Literature Cited
- Bartnicki-Garcia S (1973) Fundamental aspects of hyphal morphogenesis. In: Ashworth JM, Smith JE (eds) Microbial differentiation: Symposium 23 of the Society for General Microbiology. Cambridge: Cambridge University Press, pp 245–267
Google Scholar - Bartnicki-Garcia S, Nickerson WJ (1962) Nutrition, growth and morphogenesis of_Mucor rouxii_. J. Bacteriol 84:841–858
PubMed Google Scholar - Cabib E (1987) The synthesis and degradation of chitin. Adv Enzymol 59:59–101
PubMed Google Scholar - Cano C, Ruiz-Herrera J (1988) Developmental stages during the germination of_Mucor._ sporangiospores. Exp Mycol 12:47–69
Google Scholar - Chagolla A, Pedraza M, Lopez-Romero E (1987) Actividad quitinolitica en extractos libres de celulas miceliales de_Mucor rouxii_. Rev Mex Micol 3:283–292
Google Scholar - Gooday GW, Humphreys AM, McIntosh WH (1986) Roles of chitinases in fungal growth. In: Muzzarelli R, Jeuniaux C, Gooday GW (eds) Chitin in nature and technology. New York: Plenum Press, pp 83–91
Google Scholar - Lopez-Romero E, Ruiz-Herrera J, Bartnicki-Garcia S (1982) The inhibitory protein of chitin synthetase from_Mucor rouxii_ is a chitinase. Biochim Biophys Acta 702:233–236
PubMed Google Scholar - Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
PubMed Google Scholar - Pedraza-Reyes M, Lopez-Romero E (1989) Purification and some properties of two forms of chitinase from mycelial cells of_Mucor rouxii_. J Gen Microbiol 135:211–218
PubMed Google Scholar - Rosenberger RF (1979) Endogenous lytic enzymes and wall metabolism. In: Burnett JH, Trinci APJ (eds) Fungal walls and hyphal growth. Cambridge: Cambridge University Press pp 265–277
Google Scholar - Wessels JGH (1986) Cell wall synthesis in apical hyphal growth. Int Rev Cytol 104:37–79
Google Scholar
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Authors and Affiliations
- Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, Gto. 36000, Guanajuato, Mexico
Mario Pedraza-Reyes & Everardo Lopez-Romero - Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del IPN, Apartado Postal 14-740, 07000, Mexico, Mexico
Everardo Lopez-Romero
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- Mario Pedraza-Reyes
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Pedraza-Reyes, M., Lopez-Romero, E. Detection of nine chitinase species in germinating cells of_Mucor rouxii_.Current Microbiology 22, 43–46 (1991). https://doi.org/10.1007/BF02106211
- Issue Date: January 1991
- DOI: https://doi.org/10.1007/BF02106211