Interpretive criteria for temocillin disk diffusion susceptibility testing (original) (raw)

Abstract

The susceptibility of 677 clinical bacterial isolates to temocillin was determined by broth microdilution and disk diffusion methods for the purpose of evaluating disks with three different temocillin concentrations and determining the temocillin disk diffusion interpretive criteria. The 60_μ_g temocillin disk provided the highest interpretive accuracy (96.2 %), although the 30_μ_g disk differed by having only three (1.2 %) additional minor interpretive errors. Based on available temocillin pharmacokinetics and recommended dosage schedules, the minimum inhibitory concentration breakpoints chosen were: ⩾ 32_μ_g/ml = resistant and ⩽ 16_μ_g/ml = susceptible. The corresponding disk diffusion zone diameter breakpoints for the 60_μ_g disk were ⩽ 17 mm and ⩾ 21 mm; zone diameters of 18–20 mm were considered intermediate. For the 30_μ_g disk these were ⩽ 15 mm, ⩾ 19 mm and 16–18 mm respectively.

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Authors and Affiliations

  1. Department of Pathology, St. Vincent Hospital and Medical Center, 97225, Portland, Oregon, USA
    P. C. Fuchs
  2. Clinical Microbiology Institute, 97062, Tualatin, Oregon, USA
    A. L. Barry
  3. Antimicrobics and Resistance Mechanisms Branch, Centers for Disease Control, 30333, Atlanta, Georgia, USA
    C. Thornsberry
  4. Department of Pathology, Kaiser Foundation Laboratories (Oregon Region), 97015, Clackamas, Oregon, USA
    R. N. Jones

Authors

  1. P. C. Fuchs
  2. A. L. Barry
  3. C. Thornsberry
  4. R. N. Jones

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Fuchs, P.C., Barry, A.L., Thornsberry, C. et al. Interpretive criteria for temocillin disk diffusion susceptibility testing.Eur. J, Clin. Microbiol. 4, 30–33 (1985). https://doi.org/10.1007/BF02148656

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