High-frequency transformation ofStaphylococcus aureus by electroporation (original) (raw)
Abstract
Plasmids carrying resistances for a variety of antibiotics were successfully transformed into_Staphylococcus aureus_ by electroporation. When frozen cell preparations were used, the procedure could be carried out in as little as 30 min. Frequencies as high as 7.6 × 105 transformants/µg of DNA were obtained with DNA isolated from both large-scale plasmid preparations and from minilysate preparations. A procedure was also developed for preparation and freezing (−70°C) of electrocompetent cells. These preparations have remained electrotransformable for 2 months without loss of activity.
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- Department of Pathology, Kansas State University, 66506, Manhattan, KS, USA
Ginger Rhoads Kraemer & John J. Iandolo
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- Ginger Rhoads Kraemer
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Kraemer, G.R., Iandolo, J.J. High-frequency transformation of_Staphylococcus aureus_ by electroporation.Current Microbiology 21, 373–376 (1990). https://doi.org/10.1007/BF02199440
- Issue Date: December 1990
- DOI: https://doi.org/10.1007/BF02199440