High-frequency transformation ofStaphylococcus aureus by electroporation (original) (raw)

Abstract

Plasmids carrying resistances for a variety of antibiotics were successfully transformed into_Staphylococcus aureus_ by electroporation. When frozen cell preparations were used, the procedure could be carried out in as little as 30 min. Frequencies as high as 7.6 × 105 transformants/µg of DNA were obtained with DNA isolated from both large-scale plasmid preparations and from minilysate preparations. A procedure was also developed for preparation and freezing (−70°C) of electrocompetent cells. These preparations have remained electrotransformable for 2 months without loss of activity.

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Authors and Affiliations

  1. Department of Pathology, Kansas State University, 66506, Manhattan, KS, USA
    Ginger Rhoads Kraemer & John J. Iandolo

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  1. Ginger Rhoads Kraemer
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  2. John J. Iandolo
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Kraemer, G.R., Iandolo, J.J. High-frequency transformation of_Staphylococcus aureus_ by electroporation.Current Microbiology 21, 373–376 (1990). https://doi.org/10.1007/BF02199440

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