Effects of acute and chronic desipramine treatment on somatostatin receptors in brain (original) (raw)
Abstract
The effects of acute (5 mg/kg, IP twice daily for 2 days) and chronic (5 mg/kg IP twice daily for 21 days) administration of desipramine (DMI) on [125I]-Tyr11-somatostatin binding sites in brain were examined. There was no change in [125I]Tyr11-somatostatin binding in membranes prepared from the frontal cortex, striatum, and hippocampus of rats acutely or chronically treated with DMI as compared to non treated animals. [125I]Tyr11-Somatostatin binding was increased in membranes prepared from the rat nucleus accumbens only after chronic DMI administration. Scatchard analysis of the binding data from the nucleus accumbens showed that [125I]Tyr11-somatostatin labels a single population of somatostatin binding sites with an affinity constant, Kd, of 1.8±0.60 nM and a Bmax of 330±90 fmol/mg protein. Chronic treatment with DMI increased the Bmax (500±140 fmol/mg protein) but had no effect on the Kd. This finding shows a regional effect of DMI on [125I]Tyr11-somatostatin binding sites in rat brain and suggests that somatostatin may play a role in the pathophysiology of depression.
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Authors and Affiliations
- Laboratory of Pharmacology, Department of Basic Sciences, Division of Medicine, School of Health Sciences, University of Crete, 71409, Heraklion, Greece
Evanghelia G. Gheorvassaki, Kyriaki Thermos, George Liapakis & Christina Spyraki
Authors
- Evanghelia G. Gheorvassaki
- Kyriaki Thermos
- George Liapakis
- Christina Spyraki
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Gheorvassaki, E.G., Thermos, K., Liapakis, G. et al. Effects of acute and chronic desipramine treatment on somatostatin receptors in brain.Psychopharmacology 108, 363–366 (1992). https://doi.org/10.1007/BF02245124
- Received: 29 November 1991
- Revised: 21 February 1992
- Issue date: August 1992
- DOI: https://doi.org/10.1007/BF02245124