Comparative FISH mapping of bovine cosmids to reindeer chromosomes demonstrates conservation of the X-chromosome (original) (raw)

Abstract

Three X chromosome-specific bovine cosmids were used for fluorescence_in situ_ hybridization mapping on reindeer (Rangifer tarandus) chromosomes, to test whether such large genomic clones could be used for comparative mapping across distantly related species. All three cosmids showed distinct unique hybridization sites on the reindeer X. Comparative map locations of these cosmids, together with the relative C-banding and genome size data on the X chromosomes of the two species, provide preliminary indications that the short and long arms of bovine X correspond, respectively, to the long and short arms of the reindeer X. The study also demonstrates that cosmid clones can be used successfully for comparative mapping across species that diverged 35 million years ago.

Access this article

Log in via an institution

Subscribe and save

• Starting from 10 chapters or articles per month
• Access and download chapters and articles from more than 300k books and 2,500 journals
• Cancel anytime View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

• Chowdhary BP, de la Sena C, Harbitz I, Eriksson I, Gustavsson I (1995) FISH on metaphase and interphase chromosomes demonstrates the physical order of the genes for GPI, CRC and LIPE in pigs.Cytogenet Cell Genet 71: 175–178.
  PubMed Google Scholar
• Colbert EH (1969)Evolution of the Vertebrates: a History of the Backboned Animals through Time. New York: John Wiley & Sons.
  Google Scholar
• Fraccaro M, Gustavsson I, Hulten Maj, Lindsten J, Tiepolo L (1968) Chronology of DNA replication in the sex chromosomes of the reindeer (Rangifer tarandus L).Cytogenetics 7: 96–211.
  Google Scholar
• Gripenberg U (1984) Characterization of the karyotype of the reindeer (Rangifer tarandus); the distribution of the heterochromatin in the Reindeer and the Scandinavian moose (Alces alces).Proc of the 6th Eur Colloq Cytogenet Domest Anim 1984, pp 68–79.
• Gripenberg U, Wessman M (1993) Restriction endonuclease staining profiles in the C-heterochromatin of Cervidae. II. The sex chromosomes.Hereditas 118: 251–257.
  Article Google Scholar
• Makino S (1944) A contribution to the study of the chromosomes of domestic mammals.Zool Mag (Tokyo)56: 8–15.
  Google Scholar
• Matthey R (1945) L'évolution de formule chromosomiale chez les vertébrés.Experientia 1: 50–56, 78–86.
  Article Google Scholar
• Lin CC, Newton DR, Church RB (1977) Identification and nomenclature for G-banded bovine chromosomes.Can J Genet Cytol 19: 271–282.
  Google Scholar
• Nes N, Armud J, Tondevold OB (1965) Chromosome studies in reindeer (Rangifer tarandus).Nord Vet Med 17: 589–593.
  Google Scholar
• Ohno S (1967)Sex Chromosomes and Sex Linked Genes. Berlin: Springer.
  Google Scholar
• Ohno S (1969) The mammalian genome in evolution and conservation of the original X-linkage group, in Benirshke K, ed.Comparative Mammalian Cytogenetics. New York: Springer.
  Google Scholar
• Ohno S, Becak W, Becak ML (1964) X-autosome ratio and the behaviour pattern of individual X chromosomes in placental mammals.Chrosoma (Berlin)15: 14–30.
  Article Google Scholar
• Pathak S, Stock AD (1974) The X chromosomes of mammals: karyological homology as revealed by banding techniques.Genetics 78: 703–714.
  PubMed Google Scholar
• Popescu CP (1969) Ideogram of yak (Bos grunniens), cattle (Bos taurus) and their hybrids.Ann Genet Sel Anim 1: 207–217.
  Google Scholar
• Seabright M (1971) A rapidly banding technique for human chromosomes.Lancet 2: 971–972.
  Article Google Scholar
• Scherthan H, Cremer T, Arnason U et al. (1994) Comparative chromosome painting discloses homologous segments in distantly related mammals.Nature Genet 6: 342–347.
  Article PubMed Google Scholar

Download references

Author information

Authors and Affiliations

1. Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, S-750 07, Uppsala, Sweden
   B. Prakash, I. Gustavsson & B. P. Chowdhary
2. Department of Applied Zoology, University of Kuopio, Box 1627, SF-70211, Kuopio, Finland
   V. Kuosku
3. Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Box 8146, N-0033, Oslo, Norway
   I. Olsaker

Authors

1. B. Prakash
2. V. Kuosku
3. I. Olsaker
4. I. Gustavsson
5. B. P. Chowdhary

Corresponding author

Correspondence toB. P. Chowdhary.

Additional information

accepted for publication by M. Schmid

Rights and permissions

About this article

Cite this article

Prakash, B., Kuosku, V., Olsaker, I. et al. Comparative FISH mapping of bovine cosmids to reindeer chromosomes demonstrates conservation of the X-chromosome.Chromosome Res 4, 214–217 (1996). https://doi.org/10.1007/BF02254962

Download citation

• Received: 16 November 1995
• Revised: 11 January 1996
• Accepted: 12 January 1996
• Issue date: April 1996
• DOI: https://doi.org/10.1007/BF02254962

Key words