The murine homolog of the human breast and ovarian cancer susceptibility geneBrca1 maps to mouse chromosome 11D (original) (raw)

Abstract

The recently cloned human breast and ovarian cancer suseptibility gene,BRCA1, is located on human chromosome 17q21. We have isolated murine genomic clones containing_Brca1_ as a first step in generating a mouse model for the loss of_BRCA1_ function. A mouse genomic library was screened using probes corresponding to exon 11 of the human_BRCA1_ gene. Two overlapping mouse clones were identified that hybridized to human_BRCA1_ exons 9–12. Sequence analysis of 1.4 kb of the region of these clones corresponding to part of human exon 11 revealed 72% nucleic acid identity but only 50% amino acid identity with the human gene. The longest of the mouse_Brca1_ genomic clones maps to chromosome 11D, as determined by two-color fluorescence in situ hybridization. The synteny to human chromosome 17 was confirmed by cohybridization with the mouse probe for the NF1-gene. This comparative study confirms that the relative location of the_BRCA1_ gene has been conserved between mice and humans.

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Authors and Affiliations

1. Diagnostic Development Branch, National Center for Human Genome Research/NIH, 20892-4470, Bethesda, MD, USA
   Evelin Schröck & Thomas Ried
2. Laboratory of Genetic Disease Research, Natinal Center for Human Genome Research/NIH, 20892-4470, Bethesda, MD, USA
   Denise Larson & Anthony Wynshaw-Boris
3. Laboratory of Gene Transfer, National Center for Human Genome Research/NIH, Bldg. 49, Rm. 3A14, 49 Convent Drive, 20892-4470, Bethesda, MD, USA
   Priscilla Badger, Michael Erdos & Lawrence Brody

Authors

1. Evelin Schröck
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2. Priscilla Badger
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3. Denise Larson
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4. Michael Erdos
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5. Anthony Wynshaw-Boris
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6. Thomas Ried
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7. Lawrence Brody
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Schröck, E., Badger, P., Larson, D. et al. The murine homolog of the human breast and ovarian cancer susceptibility gene_Brca1_ maps to mouse chromosome 11D.Hum Genet 97, 256–259 (1996). https://doi.org/10.1007/BF02265277

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• Received: 21 August 1995
• Revised: 29 September 1995
• Issue Date: February 1996
• DOI: https://doi.org/10.1007/BF02265277

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