Calcium binding by chondroitin sulfate (original) (raw)
Abstract
The binding of calcium by chondroitin sulfate C was studied by employing biological and physicochemical methods for the quantitative determination of ionic calcium. Mean values for log_K_ j, the logarithm of the uncorrected formation constant for a calcium-chondroitin sulfate complex, were 1.64 with the frog heart technique, 1.55 with the murexide method, 1.39 with ultrafiltration and 1.04 with the calcium selective electrode. The magnitude of these values suggests that chondroitin sulfate has a relatively high binding capacity for calcium, but calculated values for an exchange parameter_K_ p indicate that calcium has a low affinity for the polysaccharide.
Résumé
La liaison du calcium par le sulfate de chondroitin C a été étudiée en utilisant des méthodes biologiques et physicochimiques pour la détermination quantitative du calcium ionique. Les valeurs moyennes pour log_K_ j, le logarithme de la constante de formation non corrigée d'un complexe calcium-sulfate de chondroitin C, sont 1,64 par la technique de cœur de grenouille, 1,55 par la méthode de murexide, 1,39 par l'ultrafiltration et 1,04 par l'électrode sélective du calcium. L'ordre de grandeur de ces valeurs suggèrent que le sulfate de chondroitin C a une capacité de liaison pour calcium relativement élevée, mais les valeurs calculées pour un paramètre d'échange_K_ p indiquent que le calcium a une affinité faible pour le polysaccharide.
Zusammenfassung
Die Bindung von Calcium mit Chondroitinsulfat C wurde untersucht mittels biologischer und physikalisch-chemischer Methoden für die quantitative Bestimmung des ionischen Calciums. Im Durchschnitt ergab log_K_ j, der Logarithmus der unkorrigierten Formationskonstante für einen Calciumchondroitinsulfatkomplex, 1,64 mit der Technik des Froschherzens, 1,55 mit der Murexidmethode, 1,39 mittels Ultrafiltration und 1,04 mit den Calciumselektiven Elektroden. Die Größe dieser Werte zeigt, daß Chondroitinsulfat eine relativ hohe Bindungskapazität für Calcium hat, die berechneten Werte für einen Austauschparameter_K_ p jedoch, daß Calcium eine kleine Affinität für Polysaccharide hat.
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References
- Bowness, J. M.: Calcium binding by chondroitin sulfate associated with collagen. Biochim. biophys. Acta (Amst.)58, 134–136 (1962).
Article Google Scholar - —, andK. H. Lee: Effects of chondroitin sulfates on mineralization. Biochem. J.103, 382–390 (1967).
PubMed Google Scholar - Boyd, E. S., andW. F. Neuman: The surface chemistry of bone. V. The ion-binding properties of cartilage. J. biol. Chem.193, 243–251 (1951).
PubMed Google Scholar - Buddecke, E., andR. Drzeniek: Stabilitätskonstanten der Calciumkomplexe von sauren Mucopolysacchariden. Hoppe-Seylers physiol. Chem.327, 49–64 (1962).
Google Scholar - DiSalvo, J., andM. Schubert: Specific interaction of some protein polysaccharides with freshly precipitating calcium phosphate. J. biol. Chem.242, 705–710 (1967).
PubMed Google Scholar - Dunstone, J. R.: Some cation-binding properties of cartilage. Biochem. J.72, 465–473 (1959).
PubMed Google Scholar - — Ion-exchange reactions between cartilage and various cations. Biochem. J.77, 164–170 (1960).
PubMed Google Scholar - — Ion-exchange reactions between acid mucopolysaccharides and various cations. Biochem. J.85, 336–351 (1962).
Google Scholar - Dziewiatkowski, D. D.: The role of sulfated protein-polysaccharide in calcification. Clin. Orthop. Rel. Res.35, 189–201 (1964).
Google Scholar - Farber, S. J., andM. Schubert: The binding of cations by chondroitin sulfate. J. clin. Invest.36, 1715–1722 (1957).
PubMed Google Scholar - Franek, M. D., andJ. R. Dunstone: Connective tissue protein-polysaccharides. Fractionation of the protein-polysaccharides from bovine nasal cartilage. J. biol. Chem.242, 3460–3467 (1967).
Google Scholar - Gersh, I., andH. R. Catchpole: The nature of the ground substance of connective tissue. Perspect. Biol. Med.3, 282–320 (1959–1960).
Google Scholar - Gilbert, I. G. F., andN. A. Myers: Metal binding properties of chondroitin sulfate. Biochim. biophys. Acta (Amst.)42, 469–475 (1960).
Article Google Scholar - Harnach, F., andT. B. Coolidge: Determination of ionized calcium in serum with murexide. Analyt. Biochem.6, 477–485 (1963).
Article Google Scholar - Howell, D. S.: Concepts of calcification. Arthr. and Rheum.6, 736–742 (1963).
Google Scholar - Lindenbaum, A., andK. E. Kuettner: Mucopolysaccharides and mucoproteins of calf scapula. Calc. Tiss. Res.1, 153–165 (1967).
Google Scholar - McLean, F. C., andA. B. Hastings: The state of calcium in the fluids of the body. I. The conditions affecting the ionization of calcium. J. biol. Chem.108, 285–322 (1935).
Google Scholar - Neuman, W. F., E. S. Boyd, andI. Feldman: The ion-binding properties of cartilage. Fourth Conf. Metab. Interrelations, Josiah Macy, Jr., Foundation (N.Y.)4, 100–112 (1952).
Google Scholar - Ross, J. W.: Calcium-selective electrode with liquid ion exchanger. Science156, 1378–1379 (1967).
PubMed Google Scholar - Toribara, T. Y.: Centrifuge type of ultrafiltration apparatus. Analyt. Chem.25, 1286 (1953).
Article Google Scholar - Urist, M. R. Recent advances in physiology of calcification. J. Bone Jt Surg. A46, 889–900 (1964).
Google Scholar - —, andJ. L. Abernethy: Effects of the calcium ion upon structure and calcificability of tendon. Clin. Orthop. Rel. Res.51, 255–274 (1967).
Google Scholar - —, andJ. M. Adams, Jr.: Effect of various blocking reagents upon local mechanism of calcification. Arch. Path.81, 325–342 (1966).
PubMed Google Scholar - —, andJ. M. Adams, Jr.: Calcification of tendon: A triphasic local mechanism. Arch. Path.77, 594–608 (1964).
PubMed Google Scholar - —, andF. C. McLean: Calcium oxytetracycline complexes. New apparatus for frog heart method of estimation of calcium ion concentration. Proc. Soc. exp. Biol. (N.Y.)109, 801–804 (1962).
Google Scholar - Weinstock, A., P. C. King, andR. E. Wuthier: The ion-binding characteristics of reconstituted collagen. Biochem. J.102, 983–988 (1967).
Google Scholar
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Authors and Affiliations
- Bone Research Laboratory, School of Medicine, University of California, 1000 Veteran Avenue, 90024, Los Angeles, California
Marshall R. Urist M.D., Donald P. Speer, Kenneth J. Ibsen & Basil S. Strates
Authors
- Marshall R. Urist M.D.
- Donald P. Speer
- Kenneth J. Ibsen
- Basil S. Strates
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Urist, M.R., Speer, D.P., Ibsen, K.J. et al. Calcium binding by chondroitin sulfate.Calc. Tis Res. 2, 253–261 (1968). https://doi.org/10.1007/BF02279213
- Received: 04 April 1968
- Issue date: December 1968
- DOI: https://doi.org/10.1007/BF02279213