Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin (original) (raw)
Summary
The high-molecular-weight (HMW) subunits of glutenin from about 185 varieties were fractionated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). About 20 different, major subunits were distinguished by this technique although each variety contained, with only a few exceptions, between 3 and 5 subunits. Further inter-varietal substitution lines to those already described (Payne et al. 1980) were analysed and the results indicate that all the HMW subunits are controlled by the homoeologous group 1 chromosomes. All hexaploid varieties studied except ‘NapHal’ contained two major subunits controlled by chromosome 1D. Their genes were shown to be tightly linked genetically for only four different types of banding patterns were observed. The nominal molecular weights determined after fractionation in 10% polyacrylamide gels were between 110,000 and 115,000 for the larger of the two subunits and between 82,000 and 84,000 for the smaller. One quarter of the varieties contained only one major HMW subunit controlled by chromosome 1B whereas the rest had two. The chromosome 1B subunits were the most varied and nine different banding patterns were detected. All the subunits had mobilities which were intermediate between those of the two chromosome 1D-controlled subunits. Only two types of HMW subunit controlled by chromosome 1A were detected in all the varieties examined; a single variety never contained both of these subunits and 40% of varieties contained neither. The chromosome 1A-controlled subunits had slightly slower mobilities in 10% gels than the largest HMW subunit controlled by chromosome 1D. About 100 single grains were analysed from each of five different crosses of the type (F1 of variety A × variety B) × variety C. The results indicate that the genes on chromosome 1B which control the synthesis of subunits 6, 7, 13, 14 and 17 are allelic, as are the genes of the chromosome 1A-controlled subunits, 1 and 2.
Access this article
Subscribe and save
- Starting from 10 chapters or articles per month
- Access and download chapters and articles from more than 300k books and 2,500 journals
- Cancel anytime View plans
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
Literature
- Bietz, J.A.; Wall, J.S. (1973): Isolation and characterization of gliadin-like subunits from glutenin. Cereal Chemistry50, 537–547
CAS Google Scholar - Blakesley, R.W.; Boezi, J.A. (1977): A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G250. Analyt. Biochem.82, 580–582
Article CAS PubMed Google Scholar - Brown, J.W.S.; Kemble, R.J.; Law, C.N.; Flavell, R.B. (1979): Control of endosperm proteins in_Triticum aestivum_ (var. ‘Chinese Spring’) and_Aegilops umbellulata_ by homoeologous group 1 chromosomes. Genetics93, 189–200
CAS PubMed Central PubMed Google Scholar - Brown, J.W.S.; Flavell, R.B. (1981): Fractionation of wheat gliadin and glutenin subunits by two-dimensional electrophoresis and the role of the group 6 and group 2 chromosomes in gliadin synthesis. Theor. Appl. Genet. (in press)
- Caldwell, K.A.; Kasarda, D.D. (1978): Assessment of genomic and species relationships in_Triticum_ and_Aegilops_ by PAGE and by differential staining of seed albumins and globulins. Theor. Appl. Genet.52, 273–280
Article CAS PubMed Google Scholar - Danno, G.; Kanazawa, K.; Natake, M. (1978): Improved fractionation of constituent polypeptides from wheat glutenin. Agric. Biol. Chem.42, 11–16
Article CAS Google Scholar - Huebner, F.R.; Wall, J.S. (1974): Wheat glutenin subunits I Preparative separation by gel filtration and ion-exchange chromatography. Cereal Chemistry51, 228–240
CAS Google Scholar - Khan, K.; Bushuk, W. (1979): Studies of glutenin. 13. Gel filtration, isoelectric focusing, and amino acid composition studies. Cereal Chemistry56, 505–512
CAS Google Scholar - Kimber, G.; Athwal, R.S. (1972): A reassessment of the course of evolution in wheat. Proc. Natn. Acad. Sci. USA.69, 912–915
Article CAS Google Scholar - O'Farrell, P.H. (1975): High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem.250, 4007–4021
PubMed Central PubMed Google Scholar - O'Farrell, P.Z.; Goodman, H.M.; O'Farrell, P.H. (1977): High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell12, 1133–1142
Article PubMed Google Scholar - Payne, P.I.; Corfleld, K.G. (1979): Subunit composition of wheat glutenin proteins, isolated by gel filtration in a dissociating medium. Planta145, 83–88
Article CAS PubMed Google Scholar - Payne, P.I.; Law, C.N; Mudd, E.E. (1980): Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor. Appl. Genet.58, 113–120
Article CAS PubMed Google Scholar - Payne, P.I.; Corfield, K.G.; Holt, L.M.; Blackman, J.A. (1981): Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. J. Sci. Food Agric.32, 51–60
Article CAS Google Scholar - Payne, P.I.; Holt, L.M.; Law, C.N. (1981): Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. 1. Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor. Appl. Genet. 000
- Willson, D.L.; Hall, M.E.; Stone, G.C.; Rubin, R.W. (1977): Some improvements in two-dimensional gel electrophoresis of proteins. Analyt. Biochem.83, 32–44
Google Scholar
Author information
Authors and Affiliations
- Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, England
P. I. Payne, L. M. Holt & C. N. Law
Authors
- P. I. Payne
- L. M. Holt
- C. N. Law
Additional information
Communicated by R. Riley
Rights and permissions
About this article
Cite this article
Payne, P.I., Holt, L.M. & Law, C.N. Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin.Theoret. Appl. Genetics 60, 229–236 (1981). https://doi.org/10.1007/BF02342544
- Received: 10 April 1981
- Published: 21 February 2006
- Issue date: October 1981
- DOI: https://doi.org/10.1007/BF02342544