Genetic evidence for the existence of cryptic species in theAnopheles albitarsis complex in Brazil: Allozymes and mitochondrial DNA restriction fragment length polymorphisms (original) (raw)
Abstract
Allozyme and mitochondrial DNA (mtDNA) restriction studies were undertaken to determine the extent of genetic divergence among field populations of_Anopheles albitarsis_ in Brazil. Two sympatric species,An. deaneorum and_An. marajoara_, were identified in collections from Costa Marques (CM), Rondonia. Genetic evidence includes (1) the presence of two types of individuals, each with diagnostic allelic clusters (for_Had-1, Pgi-1, Pep-1, Mpi-1_, and_Idh-1_), (2) a deficiency of heterozygotes, and (3) characteristic mtDNA haplotypes. In addition, two allopatric cryptic species of_An. marajoara_ were identified, one from Iguape (An. marajoara form IG), Sao Paulo state, and the other from the Island of Marajo (An. marajoara form MA). Though form IG and form-MA resemble form CM in wing spot morphology, they differ from it in diagnostic allozymes and mtDNA haplotypes.An. marajoara form CM had a higher variability (mean heterozygosity,H_=0.22, and percentage of polymorphic loci,P_=66.7) than did form IG and form MA (H_=0.08 in both, and_P_=25.0 and 33.3, respectively). Form MA and form IG are genetically more similar to each other than both are to form CM. Based on wing morphology, estimates of_F statistics, and genetic similarities, we propose that_An. albitarsis in Brazil is a species complex. It comprises at least two morphologically distinguishable species: (1)An. deaneorum (currently one taxon) and (2) the_An. marajoara species complex, which further consists of at least three cryptic forms,marajoara form MA,marajoara form IG, and_marajoara_ form CM.
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Authors and Affiliations
- Biosciences Research Laboratory, USDA, Agricultural Research Searvice, SU Station, P.O. Box 5674, 58105, Fargo, North Dakota
S. K. Narang - Department of Entomology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, 20307-5100, Washington, D.C.
T. A. Klein - Medical and Veterinary Entomology Laboratory, USDA, Agricultural Service, P.O. Box 14565, 32604, Gainesville, Florida
O. P. Perera - Instituto de Biologia do Exercito, Rua Francisco Manuel, 102 Bonfica, CEP 20.911, Rio de Janeiro, R.J., Brasil
José Bento Lima - Laboratorio Central de Entomologia, Divisao de Malaria, SUCAM, Ministerio de Saude, CEP 70.058, Brasilia, DF, Brasil
Amazonia Toda Tang
Authors
- S. K. Narang
- T. A. Klein
- O. P. Perera
- José Bento Lima
- Amazonia Toda Tang
Additional information
The views of the authors do not purport to reflect the position of the Department of the Army or the Department of Defense of the United States.
This research was conducted when the senior author was on the staff of the USDA-ARS Laboratory in Gainesville, Florida.
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Narang, S.K., Klein, T.A., Perera, O.P. et al. Genetic evidence for the existence of cryptic species in the_Anopheles albitarsis_ complex in Brazil: Allozymes and mitochondrial DNA restriction fragment length polymorphisms.Biochem Genet 31, 97–112 (1993). https://doi.org/10.1007/BF02399823
- Received: 30 January 1992
- Revised: 30 January 1992
- Issue date: February 1993
- DOI: https://doi.org/10.1007/BF02399823