Segregation of fate during cleavage of frog (Xenopus laevis) blastomeres (original) (raw)
Summary
A detailed fate map of all the progeny derived from each of the blastomeres of the 4- and 8-cell stage South African clawed frog (Xenopus laevis) embryo is presented. Each “identified” blastomere that results from stereotypic cleavages has a characteristic set of progeny that distinguishes it from the other blastomeres of the embryo. The 4-cell dorsal (D) blastomere is the major progenitor of the stomodeum, cement gland, retina, notochord, head somite, pharynx and liver. The 4-cell ventral (V) blastomere is the major progenitor of the trunk and fin epidermis, ventral somite, nephrotome, lateral plate mesoderm and proctodeum. The other organs are derived from both blastomeres. At the next cell division, the animal hemisphere daughters of both blastomeres (D1 and V1, respectively) become the major progenitors for head ectodermal and mesodermal structures, and the vegetal hemisphere daughters become the major progenitors for trunk mesodermal (D2) or trunk endodermal (V2) structures. Semiquantitative lineage diagrams, using data from this and from previous studies demonstrate that as cleavage proceeds from the 2- to the 32-cell stage, the progenitors for particular organs or for specific regions of organs segregate into defined regions of the blastula. To determine whether this segregation is related to the position of the blastomere or to its geneological lineage, we compared the fates of radial 8-cell blastomeres to those of stereotypic 8-cell blastomeres. Radial blastomeres have fates nearly equivalent to the sum of the two 16-cell blastomeres that occupy the same position in the embryo, demonstrating that fate depends upon blastomere position rather than lineage.
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References
- Heasman J, Wylie CC, Hausen P, Smith JC (1984) Fates and states of determination of single vegetal pole blastomeres of_Xenopus laevis_. Cell 37:185–194
Article PubMed CAS Google Scholar - Hirose G, Jacobson M (1979) Clonal organization of the central nervous system of the frog. I. Clones stemming from individual blastomeres of the 16-cell and earlier stages. Dev Biol 71:191–202
PubMed CAS Google Scholar - Jacobson M (1985) Clonal analysis and cell lineages of the vertebrate central nervous system. Annu Rev Neurosci 8:71–102
Article PubMed CAS Google Scholar - Jacobson M, Hirose G (1981) Clonal organization of the central nervous system of the frog. II. Clones stemming from individual blastomeres of the 32- and 64-cell stages. J Neurosci 1:271–284
PubMed CAS Google Scholar - Jacobson M, Wei-long X (1989) States of determination of single cells transplanted between 512-cell_Xenopus_ embryos. Dev Biol 131:119–125
PubMed CAS Google Scholar - Jones EA, Woodland HR (1987) The development of animal cap cells in_Xenopus_: a measure of the start of animal cap competence to form mesoderm. Development 101:557–563
Google Scholar - Kageura H, Yamana K (1983) Pattern regulation in isolated halves and blastomeres of early_Xenopus laevis_. J Embryol Exp Morphol 74:221–234
PubMed CAS Google Scholar - Kageura H, Yamana K (1984) Pattern regulation in defect embryos of_Xenopus laevis_. Dev Biol 101:410–415
Article PubMed CAS Google Scholar - Kimmel CB, Law RD (1985) Cell lineage of zebrafish blastomeres. III. Clonal analysis of the blastula and gastrula stages. Dev Biol 108:94–101
Article PubMed CAS Google Scholar - Klein SL (1987) The first cleavage furrow demarcates the dorsalventral axis in_Xenopus laevis_ embryos. Dev Biol 120:299–304.
Article PubMed CAS Google Scholar - Klein SL, Moody SA (1989) Lithium changes the ectodermal fate of individual frog blastomeres because it causes ectopic neural plate formation. Development 106:599–610
PubMed CAS Google Scholar - Kuhtreiber WM, Serras F, van den Biggelaar JAM (1987) Spreading of microinjected horseradish peroxidase to nondescendant cells in embryos of_Patella_ (Molusca, Gastropoda). Development 100:713–722
Google Scholar - Masho R (1988) Fates of animal dorsal blastomeres of eight-cell stage_Xenopus_ embryos vary according to the specific patterns of the third cleavage plane. Dev Growth Differ 30:347–359
Article Google Scholar - Masho R (1990) Close correlation between the first cleavage plane and the body axis in early_Xenopus_ embryos. Dev Growth Differ 32:57–64
Article Google Scholar - Masho R, Kubota HY (1986) Developmental fates of blastomeres of eight-cell stage_Xenopus laevis_ embryos. Dev Growth Differ 28:113–123
Article Google Scholar - Moody SA (1987a) Fates of the blastomeres of the 16-cell_Xenopus_ embryo. Dev Biol 119:560–578
Article PubMed CAS Google Scholar - Moody SA (1987b) Fates of the blastomeres of the 32-cell_Xenopus_ embryo. Dev Biol 122:300–319
Article PubMed CAS Google Scholar - Moody SA (1989) Quantitative lineage analysis of the origin of frog primary motor and sensory neurons from cleavage stage blastomeres. J Neurosci 9:2919–2930
PubMed CAS Google Scholar - Newport J, Kirschner M (1982) A major developmental transition in early_Xenopus_ embryos. I. Characterization and time of cellular changes at the midblastula stage. Cell 30:675–686
Article PubMed CAS Google Scholar - Nieuwkoop PD, Faber J (1964) Normal Table of_Xenopus laevis_ (Daudin). North-Holland, Amsterdam
Google Scholar - Nishida H, Satoh N (1983) Cell lineage analysis in Ascidian embryos by intracellular injection of a tracer enzyme. I. Up to the eight-cell stage. Dev Biol 99:382–394
Article PubMed CAS Google Scholar - Slack JMW (1983) From Egg to Embryo, Cambridge Univ Press, London
Google Scholar - Stent GS (1985) The role of cell lineage in development. Philos Trans R Soc Lond [Biol] 312:3–19
CAS Google Scholar - Stent GS, Weisblat DA (1985) Cell lineage in the development of invertebrate nervous systems. Annu Rev Neurosci 8:45–70
Article PubMed CAS Google Scholar - Sulston JE, Schierenberg E, White JG, Thomson JN (1983) The embryonic cell lineage of the nematode_Caenorhabditis elegans_. Dev Biol 100:64–119
Article PubMed CAS Google Scholar - Weisblat DA, Harper G, Stent GS, Sawyer RT (1980) Embryonic cell lineages in the nervous system of the glossiphoniid leech_Helobdella triserialis_. Dev Biol 76:58–78
Article PubMed CAS Google Scholar - Weisblat DA, Kim SY, Stent GS (1984) Embryonic origins of cells in the leech_Helobdella triserialis_. Dev Biol 104:64–85
Article Google Scholar - Ziomek CA, Johnson MH, Handyside AH (1982) The developmental potential of mouse 16-cell blastomeres. J Exp Zool 221:345–355
Article PubMed CAS Google Scholar
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Authors and Affiliations
- Department of Anatomy and Cell Biology, University of Virginia, Health Sciences Center, 22908, VA, Charlottesville, USA
Sally A. Moody & Michael J. Kline
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- Sally A. Moody
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Moody, S.A., Kline, M.J. Segregation of fate during cleavage of frog (Xenopus laevis) blastomeres.Anat Embryol 182, 347–362 (1990). https://doi.org/10.1007/BF02433495
- Accepted: 25 June 1990
- Issue Date: October 1990
- DOI: https://doi.org/10.1007/BF02433495