Effects of double bond configuration on lecithin synthesis (original) (raw)

Abstract

Stearyl-CoA, oleyl-CoA and elaidyl-CoA were prepared and tested as substances in synthesizing lecithin from 1- and 2-acyglycerol-3-phosphorylcholine. The rates of acyltransfer were followed by a continuous spectrophotometric assay. The enzyme(s) used to catalyze esterification at the 2-position did not discriminate appreciably between the_cis_ and_trans_ isomers of octadecenoate and transferred them more rapidly than the saturated acid, octadecanoate. However, the enzyme(s) which acted at the 1-position discriminated markedly between the geometric isomers and rapidly transferred to_trans_- octadecenoate and octadecanoate.

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Authors and Affiliations

1. Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan
   W. E. M. Lands

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Lands, W.E.M. Effects of double bond configuration on lecithin synthesis.J Am Oil Chem Soc 42, 465–467 (1965). https://doi.org/10.1007/BF02540085

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• Received: 04 January 1965
• Accepted: 24 February 1965
• Issue date: June 1965
• DOI: https://doi.org/10.1007/BF02540085

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