An easy technique for the clearing of histochemically stained plant tissue (original) (raw)

Abstract

The sites of GUS-activity in strongly pigmented whole-mount preparations or thick handmade sections of plant material can easily be visualized by clearing it with chlorallactophenol (CLP), without affecting the ClBr-indigo precipitate.

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Abbreviations

GUS:

β-glucuronidase

X-gluc:

5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid

CLP:

chlorallactophenol

CIBr-indigo:

dichloro-dibromoindigo

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Authors and Affiliations

1. Laboratorium voor morfologie, systematiek en ecologie van de planten, Universiteit Gent, B-9000, Gent, Belgium
   Tom Beeckman
2. Laboratoire Associé de l’Institut National de la Recherche Agronomique (France), Universiteit Gent, B-9000, Gent, Belgium
   Gilbert Engler

Authors

1. Tom Beeckman
2. Gilbert Engler

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Beeckman, T., Engler, G. An easy technique for the clearing of histochemically stained plant tissue.Plant Mol Biol Rep 12, 37–42 (1994). https://doi.org/10.1007/BF02668662

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• Issue date: March 1994
• DOI: https://doi.org/10.1007/BF02668662

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