PCR-mediated recombination and mutagenesis (original) (raw)

References

  1. Mullis, K. B. and Faloona, F. A. (1987) Specific synthesis of DNA in vitro via a polymerase-catalysed chain reaction.Methods Enzymol. 155, 335–350.
    Article PubMed CAS Google Scholar
  2. Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G., and Erlich, H. (1986) Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.Cold Spring Harbor Symp. Quant. Biol. 51, 263–273.
    PubMed CAS Google Scholar
  3. Kadowaki, H., Kadowaki, T., Wondisford, F. E., and Taylor, S. I. (1989) Use of polymerase chain reaction catalysed by Taq DNA polymerase for site-specific mutagenesis.Gene 76, 161–166.
    Article PubMed CAS Google Scholar
  4. Vallette, F., Mege, E., Reiss, A., and Milton, A. (1989) Constuction of mutant and chimeric genes using the polymerase chain reaction.Nucleic Acids Res. 17, 723–733.
    Article PubMed CAS Google Scholar
  5. Higuchi, R., Krummel, B., and Saiki, R.K. (1988) A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.Nucleic Acids Res. 16, 7351–7367.
    Article PubMed CAS Google Scholar
  6. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K., and Pease, L. R. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction.Gene 77, 51–59.
    Article PubMed CAS Google Scholar
  7. Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K., and Pease, L. R. (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.Gene 77, 61–68.
    Article PubMed CAS Google Scholar
  8. Horton, R. M., Cai, Z., Ho, S. N., and Pease, L. R. (1990) Gene splicing by overlap extension: tailormade genes using the polymerase chain reaction.BioTechniques 8, 528–535.
    PubMed CAS Google Scholar
  9. Kain, K. C., Orlandi, P. A., and Lanar, D. E. (1991) Universal promoter for gene expression without cloning: expression-PCR.BioTechniques 10, 366.
    PubMed CAS Google Scholar
  10. Davis, G. T., Bedzyk, W. D., Voss, E. W., and Jacobs, T. W. (1991) Single Chain Antibody (SCA) encoding genes: one-step construction and expression in eukaryotic cells.Biotechnology 9, 165–169.
    Article PubMed CAS Google Scholar
  11. Daughtery, B. L., DeMartino, J. A., Law, M.-F., Kawka, D. W., Singer, I.I., and Mark, G. E. (1991) Polymerase chain reaction facilitates the cloning, CDR-grafting, and rapid expression of a murine monoclonal antibody directed against the CD18 component of leukocyte integrins.Nucleic Acids Res. 19, 2471–2476.
    Article Google Scholar
  12. Suggs, S.V., Hirose, T., Miyake, T., Kawashima, E.H., Johnson, M. J., Itakura, K., and Wallace, R. B. (1981) Use of synthetic oligo-deoxyribonucleotides for the isolation of cloned DNA sequences, in_Developmental Biology Using Purified Genes_ ( Brown, D. D. and Fow, C. F., eds.), Academic, New York, pp. 683–693.
    Google Scholar
  13. Yon, J. and Fried, M. (1989) Precise gene fusion by PCR.Nucleic Acids Res. 17, 4895.
    Article PubMed CAS Google Scholar
  14. Sarkar, G. and Sommer, S. S. (1990) The “megaprimer” method of site-directed mutagenesis.Bio-Techniques 8, 404–407.
    CAS Google Scholar
  15. Horton, R. M. and Pease, L. R. (1991) Recombination and mutagenesis of DNA sequences using PCR, in_Directed Mutagenesis: A Practical Approach._ (McPherson, M. J., ed.) IRL, Oxford, pp. 217–247.
    Google Scholar
  16. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989)Molecular Cloning: A Laboratory Manual (2nd ed.) Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
    Google Scholar
  17. Yolov, A. A. and Shaborova, Z. A. (1990) Constructing DNA by polymerase recombination.Nucleic Acids Res. 18, 3983–3986.
    Article PubMed CAS Google Scholar
  18. Eckert, K. A. and Kunkel, T. A. (1990) High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase.Nucleic Acids Res. 18, 3739–3744.
    Article PubMed CAS Google Scholar
  19. Hanes, S. D. and Brent, R. (1991) A genetic model for interaction of the homeodomain recognition helix with DNA.Science 251, 426–430.
    Article PubMed CAS Google Scholar
  20. Rudert, R. A. and Trucco, M. (1990) DNA polymers of protein binding sequences generated by PCR.Nucleic Acids Res. 18, 6460.
    Article PubMed CAS Google Scholar
  21. Shuldiner, A. R., Scott, L. A., and Roth, J. (1990) PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.Nucleic Acids Res. 18, 1920.
    Article PubMed CAS Google Scholar
  22. Jones, D. H. and Howard, B. H. (1990) A rapid method for site-specific mutagenesis and directional subcloning by using the polymerase chain reaction to generate recombinant circles.BioTechniques 8, 178–183.
    PubMed CAS Google Scholar
  23. White, B. A. (ed.) (1993)PCR Protocols: Current Methods and Applications, Humana, Totowa, NJ.
    Google Scholar

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