Source, alterations, characteristics and use of a new dog cell line (Cf2Th) (original) (raw)

Summary

A cell line derived from normal fetal canine thymus (Cf2Th) has been in culture since 1967. During cultivation the cells have changed morphologically from a fibroblast-like to flat, fusiform appearance and karyologically from diploid (2n=78) with 76 telocentric autosomes to hypodiploid with newly formed atelocentric chromosomes. The cells retain canine characteristic enzyme activity (G6PD and LDH) as well as cell membrane fluorescence and are free of mycoplasma. High passage cells produce tumors in ATST mice. No endogenous viruses have been detected in these cells. No original publication exists, to date, on the origin of this line, but seed stocks thereof have been distributed to many laboratories and the cells have served as experimental substrates in a number of published works in oncology albeit under different designations. The present information is offered in order to establish the provenance of this valuable cell line and to list characteristics which may serve to monitor for its purity and to distinguish it from other existing cell lines of dog origin also in common use.

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Authors and Affiliations

  1. Cell Culture Laboratory, University of California, School of Public Health, Naval Biomedical Research Laboratory, 94625, Oakland, Calif.
    W. A. Nelson-Rees & R. B. Owens
  2. Viral Carcinogenesis Branch, National Cancer Institute, National Institutes of Health, Public Health Service, U. S. Department of Health, Education and Welfare, 20014, Bethesda, Md.
    P. Arnstein
  3. University of California Resources, 92037, La Jolla, Calif.
    A. J. Kniazeff

Authors

  1. W. A. Nelson-Rees
  2. R. B. Owens
  3. P. Arnstein
  4. A. J. Kniazeff

Additional information

Supported by Contract No. 1-CP-3-3237 within the Virus Cancer Program, National Cancer Institute, National Institutes of Health.

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Nelson-Rees, W.A., Owens, R.B., Arnstein, P. et al. Source, alterations, characteristics and use of a new dog cell line (Cf2Th).In Vitro Cell.Dev.Biol.-Plant 12, 665–669 (1976). https://doi.org/10.1007/BF02797468

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