Phenolic constituents of glandular trichomes onSolanum berthaultii andS. polyadenium (original) (raw)

Abstract

Phenolic compounds in Type A glandular trichomes of two insectresistant potato species were separated and identified using a combination of high pressure liquid chromatography (HPLC), UV-visible spectrometry, and1H proton nuclear magnetic resonance (NMR) procedures. Type A trichomes of_Solanum berthaultii_ and_S. polyadenium_ contained a phenolic compound tentatively identified as the glucose ester of p-hydroxyphenylpropionic acid. Its presence in trichomes of both species suggests that this compound may play a major role in formation of the brownish, insect entrapping exudate characteristic of trichome-mediated insect resistance in these species. Chlorogenic acid, a major phenolic constituent of Type A trichomes of_Solanum polyadenium_ was not detected in trichomes of 5.berthaultii. The exclusive presence of chlorogenic acid in trichome exudates of_S. polyadenium_ may be responsible, at least in part, for the greater levels of insect entrapment by this species, compared with that of_S. berthaultii._

Resumen

Se separaron e identificaron compuestos fenólicos en los tricomas glandulares Tipo A de dos especies de papa resistentes a insectos, utilizando una combinación de los métodos de cromatografía líquida de alta presión (HPLC), espectrometria visible UV, y la resonancia nuclear magnética del protón1H, (NMR). Los tricomas Tipo A de_Solanum berthaultii y S. polyadenium_ contenían un compuesto fenólico tentativamente identificado como el ester glucosado del ácido p-hidroxifenilpropiónico. Su presencia en los tricomas de ambas especies sugiere que este compuesto puede jugar un importante rol en la formación del exudado parduzco que entrampa a los insectos y que es característico de la resistencia en estas dos especies, ejercida sobre los insectos atrapados en sus tricomas. El ácido clorogénico, un constituyente principal de los tricomas Tipo A de_Solanum polyadenium_ no fue detectado en los tricomas de_Solanum berthaultii._ La presencia exclusiva del ácido clorogénico en los exudados de los tricomas de_Solanum polyadenium_ puede ser responsable, al menos en parte, de los mayores niveles de entrampamiento encontrados en esta especie, en comparación con los observados con_S. berthaultii._

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Authors and Affiliations

  1. Department of Plant Breeding, Cornell University, 14583, Ithaca, New York
    Dick A. Avé (Senior Research Associate)
  2. Department of Entomology, Cornell University, 14583, Ithaca, New York
    Ward M. Tingey (Associate Professor)

Authors

  1. Dick A. Avé
  2. Ward M. Tingey

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A publication of the Cornell University Agricultural Experiment Station, New York State College of Agriculture and Life Sciences, a Statutory College of S.U.N.Y.

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Avé, D.A., Tingey, W.M. Phenolic constituents of glandular trichomes on_Solanum berthaultii_ and_S. polyadenium_ .American Potato Journal 63, 473–480 (1986). https://doi.org/10.1007/BF02852942

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