Comparative immunostaining of T-associated plasma cells and other lymph-node cells in paraffin sections (original) (raw)

• 27 Accesses
• 24 Citations
• Explore all metrics

Summary

The immunoperoxidase technique after trypsinization was used on paraffin sections of 24 lymph nodes with reactive lymphadenitis and abudant nests of T-associated plasma cells. These cells were negative for all the markers investigated, which were: intracytoplasmic immunoglobulins (CIg), a1-antichymotrypsin(a1 -ACT), a1 -antitrypsin (a1 -AT), lysozyme(Lz) and fibronectin. Other categories of cells were positive or negative depending on their type. The best markers for polymorphs proved to be a1-AT and Lz, and for monocytes and histiocytes a1-ACT and Lz. Sinus histiocytes in particular were much more constantly and strongly positive for a1-ACT than for Lz. Endothelial cells appeared almost always positive for a1-ACT and were also occasionally positive for a1-AT. Fibronectin was consistently positive in mast cells and sometimes positive in other cells, especially those of monihistiocytic origin.

Our present findings are against a B-cell or monohistiocytic origin for T-associated plasma cells.

Access this article

Log in via an institution

Subscribe and save

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

• Alitalo K, Hovi T, Vaheri A (1980) Fibronectin is produced by human macrophages. J Exp Med 151:602–613
  Article PubMed CAS Google Scholar
• Clark RAF, Dvorak HF, Colvin RB (1981) Fibronectin in delayed-type hypersensitivity skin reactions with vessel permeability and endothelial cell activation. J Immunol 126:787–793
  PubMed CAS Google Scholar
• Isaacson P, Jones DB, Millward-Sadler GH, Judd MA, Payne S (1981) Alpha-1-antitrypsin in human macrophages. J Clin Pathol 34:982–990
  Article PubMed CAS Google Scholar
• Jaffe EA, Mosher DF (1978) Synthesis of fibronectin cultured human endothelial cells. J Exp Med 147:1779–1791
  Article PubMed CAS Google Scholar
• Kurrinen M, Vaheri A, Roberts PJ, Steman S (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 43:47–51
  Google Scholar
• Lennert K (1961) In: Uehlinger E (ed) Handbuch der Speziellen pathologischen Anatomie und Histologie I/3A. Springer, Berlin Göttingen Heidelberg
  Google Scholar
• Lennert K (1978) Malignant lymphomas other than Hodgkin’s disease. Springer, Berlin Heidelberg New York, p 33
  Google Scholar
• Lennert K (1979) Introductory remarks. In: Muller-Rucholtz W, Muller-Hermelink HK (eds) Medicine and Biology, vol 114. Plenum Press, New York and London, p 4
  Google Scholar
• Mason DY, Taylor CR (1975) The distribution of muramidase (lysozyme) in human tissues. J Clin Pathol 28:124–132
  Article PubMed CAS Google Scholar
• Mempham BL, Frater W, Mitchell BS (1979) The use of proteolytic enzymes to improve immunoglobulin staining in the PAP technique. Histochem J 11:345–357
  Article Google Scholar
• Motoi M, Stein H, Lennert K (1980) Demonstration of lysozyme, a1-anti-chymotrypsin, a1antitrypsin, albumin and transferrin with the immunoperoxidase method in lymph node cells. Virchows Arch [Cell Pathol] 35:73–82
  Article CAS Google Scholar
• Müller-Hermelink HK, Kaiserling E, Lennert K (1973) Pseudofollikulare Nester von Plasmazellen (eines besonderen Typs?) in der paracorticalen Pulpa menschlichen Lymphknoten. Virchows Archiv [Cell Pathol] 14:47–56
  Google Scholar
• Papadimitriou CS, Stein H, Papacharalmpous NX (1980) Presence of a1-anti-chymotrypsin and a1-antitrypsin in haematopoietic and lymphoid tissue cells as revealed by the immunoperoxidase method. Pathol Res Pract 169:287–297
  PubMed CAS Google Scholar
• Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spriochetes. J Histochem Cytochem 18:315–333
  PubMed CAS Google Scholar
• Vaheri A, Ruoshalti E (1975) Fibroblast surface antigen produced but not retained by virustransformed human cells. J Exp Med 142:530–543
  Article PubMed CAS Google Scholar

Download references

Author information

Authors and Affiliations

1. Department of Pathological Anatomy, School of Medicine, University of Ioannina, Ioannina, Greece
   Constantin S. Papadimitriou, Stella N. Stephanaki-Nikou, Vasiliki & D. Malamou-Mitsi

Authors

1. Constantin S. Papadimitriou
   You can also search for this author inPubMed Google Scholar
2. Stella N. Stephanaki-Nikou
   You can also search for this author inPubMed Google Scholar
3. Vasiliki
   You can also search for this author inPubMed Google Scholar
4. D. Malamou-Mitsi
   You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Papadimitriou, C.S., Stephanaki-Nikou, S.N., Vasiliki et al. Comparative immunostaining of T-associated plasma cells and other lymph-node cells in paraffin sections.Virchows Archiv B Cell Pathol 43, 31–36 (1983). https://doi.org/10.1007/BF02932940

Download citation

• Received: 13 October 1982
• Accepted: 19 January 1983
• Issue Date: December 1983
• DOI: https://doi.org/10.1007/BF02932940

Key words