Usefulness of ED036 kit for measuring serum PIVKA-II levels in small hepatocellular carcinoma (original) (raw)

Abstract

As a tumor marker for hepatocellular carcinoma (HCC), serum protein induced by vitamin K absence or antagonist-II (PIVKA-II) has high specificity, yet its sensitivity is relatively low, making it less suitable to serve as an adjunct in the diagnosis of small HCC, Recently, the ED036 kit (Eisai, Tokyo, Japan), whose detection limit is approximately ten times superior to that of a conventional kit (Eitest MONOP II, Eisai) has been developed. In this study, serum PIVKA-II levels in serum samples from 83 patients with benign chronic liver diseases (CLD) and 129 patients with HCC were measured with these two kits. With the ED036 kit, the cut-off value was set at 40 mAU/ml. For PIVKA-II measured with the ED036 kit, sensitivity was 45.0%, specificity 92.8%, and accuracy 63.7%, when we discriminated patients with HCC from those with CLD without HCC. While maintaining a high specificity, of 92.8%, the ED036 kit showed a significantly higher sensitivity than the conventional kit (45.0% versus 27.9%;P<0.0001). With patients who had HCC consisting of a single nodule 30mm or less in diameter, the positivity rate for serum PIVKA-II with the ED036 kit was significantly greater than the rate with the conventional kit (21.4% versus 9.5%;P<0.005). Thus, the ED036 kit was thought to be more useful than the conventional kit as a tumor marker for small HCC.

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References

  1. Ikeda K, Saitoh S, Koida, I, et al. Imaging diagnosis of small hepatocellular carcinoma. Hepatology 1994;20:82–87.
    Article PubMed CAS Google Scholar
  2. Hirai K, Aoki Y, Majima Y, et al. Magnetic resonance imaging of small hepatocellular carcinoma. Am J Gastroenterol 1991;86:205–209.
    PubMed CAS Google Scholar
  3. Takayasu K, Moriyama N, Muramatsu Y, et al. The diagnosis of small hepatocellular carcinomas: Efficacy of various imaging procedures in 100 patients. Am J Roentgenol 1990;155:49–54.
    CAS Google Scholar
  4. Majima Y. Growth rate of hepatocellular carcinoma by ultrasonography and its clinical significance (in Japanese with English abstract). Acta Hepatol Jap 1984;25:34–45.
    Google Scholar
  5. Weitz IC, Liebman HA. Des-γ-carboxy (abnormal) prothrombin and hepatocellular carcinoma: A critical review. Hepatology 1993;18:990–997.
    Article PubMed CAS Google Scholar
  6. Liebman HA, Furie BC, Tong M, et al. Des-γ-carboxy (abnormal) prothrombin as a serum marker of primary hepatocellular carcinoma. N Engl J Med 1984;310:1427–1431.
    PubMed CAS Google Scholar
  7. Nomura F, Ohnishi K, Tanabe Y. Clinical features and prognosis of hepatocellular carcinoma with reference to serum alphafetoprotein levels. Cancer 1989;64:1700–1707.
    Article PubMed CAS Google Scholar
  8. Taketa K. α-Fetoprotein: Reevaluation in hepatology. Hepatology 1990;12:1420–1432.
    Article PubMed CAS Google Scholar
  9. Motohara K, Kuroki Y, Kan H, et al. Detection of vitamin K deficiency by use of an enzyme-linked immunosorbent assay for circulating abnormal prothrombin. Pediatr Res 1985;19:354–357.
    PubMed CAS Google Scholar
  10. Hattori N, Ohmizo R, Unoura M, et al. PIVKA-II collaborative working group. Abnormal prothrombin measurements in hepatocellular carcinoma. J Tumor Marker Oncol 1988;3:207–216.
    Google Scholar
  11. Fujiyama S, Morishita T, Hashiguchi O, et al. Plasma abnormal prothrombin (des-γ-carboxy prothrombin) as a marker of hepatocellular carcinoma. Cancer 1988;61:1621–1628.
    Article PubMed CAS Google Scholar
  12. Taketa K, Sekiya C, Namiki M, et al. Lectin-reactive profiles of alpha-fetoprotein characterizing hepatocellular carcinoma and related conditions. Gastroenterology 1990;99:506–518.
    Google Scholar
  13. Kuromatsu R, Tanaka M, Tanikawa K. Serum alpha-fetoprotein and_Lens culinaris_ agglutinin-reactive fraction of alphafetoprotein in patients with hepatocellular carcinoma. Liver 1993;13:177–182.
    PubMed CAS Google Scholar
  14. Aoyagi Y, Isemura M, Yoshizawa Z, et al. Fucosylation of serum γ-fetoprotein in patients with primary hepatocellular carcinoma. Biochim Biophys Acta 1985;830:217–223.
    PubMed CAS Google Scholar
  15. Taketa K, Endo Y, Sekiya, C, et al. A collaborative study for the evaluation of lectin-reactive α-fetoproteins in early detection of hepatocellular carcinoma. Cancer Res 1993;53:5419–5423.
    PubMed CAS Google Scholar
  16. Yamashita F, Tanaka M, Satomura S, et al. Monitoring of lectinreactive α-fetoproteins in patients with hepatocellular carcinoma treated using transcatheter arterial embolization. Eur J Gastroenterol Hepatol 1995;7:627–633.
    PubMed CAS Google Scholar
  17. Suehiro T, Sugimachi K, Matsumata T, et al. Protein induced by vitamin K absence or antagonist II as a prognostic marker in hepatocellular carcinoma. Cancer 1994;73:2464–2471.
    Article PubMed CAS Google Scholar
  18. The Liver Cancer Study Group of Japan. The general rules of the clinical and pathological study of primary liver cancer. Jpn J Surg 1989;19:98–129.
    Article Google Scholar
  19. Thai SL, Huang GT, Yang PM, et al. Plasma des-gamma-carboxy prothrombin in the early stage of hepatocellular carcinoma. Hepatology 1990;11:481–487.
    Article Google Scholar
  20. Tanabe Y, Ohnishi K, Nomura F, et al. Plasma abnormal prothrombin levels in patients with small hepatocellular carcinoma. Am J Gastroenterol 1988;83:1386–1389.
    PubMed CAS Google Scholar
  21. Majima Y, Fujimoto T., Iwai I, et al. Histological diagnosis of hepatocellular carcinoma by a new technique of ultrasound-guided fine needle biopsy (in Japanese with English abstract). Acta Hepatol Jap 1988;29:628–636.
    Google Scholar

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Authors and Affiliations

  1. Second Department of Internal Medicine, Kurume University School of Medicine, 67 Asahi-machi, 830, Kurume, Japan
    Ryoko Kuromatsu, Masatoshi Tanaka, Yoshihiro Shimauchi, Masahiro Shimada & Kyuichi Tanikawa
  2. Eisai Company, Ltd., Tokyo, Japan
    Keisuke Watanabe & Takeshi Yokoo

Authors

  1. Ryoko Kuromatsu
  2. Masatoshi Tanaka
  3. Yoshihiro Shimauchi
  4. Masahiro Shimada
  5. Kyuichi Tanikawa
  6. Keisuke Watanabe
  7. Takeshi Yokoo

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Kuromatsu, R., Tanaka, M., Shimauchi, Y. et al. Usefulness of ED036 kit for measuring serum PIVKA-II levels in small hepatocellular carcinoma.J Gastroenterol 32, 507–512 (1997). https://doi.org/10.1007/BF02934091

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