Halothane and isoflurane enhance melanoma tumour metastasis in mice (original) (raw)

Abstract

Purpose

To investigate the incidence of tumour metastasis from B16 melanoma tumour cells in expenmental animals following exposure to equipotent concentrations of halothane or isoflurane, and to differentiate if exposure to one anaesthetic resulted in greater metastases than the other.

Methods

Experimental animals (C57B1 mice), were randomized to receive 1.3 MAC hours of halothane or isoflurane anaesthesia. The control group of animals received oxygen alone under identical conditions. Fifteen minutes after completion of anaesthesia, control and experimental groups were given 1 × 105 B16 melanoma cells intravenously. After 21 days, all animals were autopsied, and the metastatic nodules in their lungs were counted. The difference in the numbers of metastatic nodules between control and expenmental groups of animals was analyzed for significance by the Mann Whitney “U test”.

Results

More metastases were observed in the animals exposed to halothane (37.28±5.08, P< 0.0001), or isoflurane anaesthesia (28.24±4.07, P< 0.0014) than in the control animals (12.22± 1.52).

Conclusion

Exposure to halothane or isoflurane anaesthesia increased the number of pulmonary metastases in C57B1 mice compared with the control groups but there was no difference in metastases among animals treated with halothane or isoflurane.

Résumé

Objectif

Rechercher l’incidence des métastases de cellules tumorales mélanomateuses B16 sur des animaux de laboratoire après l’exposition à des concentrations équipotentes d’halothane et d’isoflurane, et vérifier si l’exposition à un anesthésique produisait un plus grand nombre de métastases qu’à l’autre.

Méthodes

Des animaux de laboratoires (souris 57BI) étaient réparties aléatoirement pour recevoir 1, 3 MAC heures d’anesthésie à l’halothane ou à l’isoflurane. Le groupe contrôle ne recevait que de l’oxygène dans des conditions expérimentales identiques. Quinze minutes après l’arrêt de l’anesthésie, les souris des groupes contrôle et expérimentaux recevaient 1 × 105 de cellules mélanomateuses B16 par la voie intraveineuse. Après 21 jours, tous les animaux étaient autopsiés et les nodules pulmonaires métastatiques dénombrés. La signification statistique de la différence entre le nombre nodules métastatiques dénombrés entre le groupe contrôle et les groupes expénmentaux était déterminée avec le test U de Mann Whitney.

Résultats

On a observé plus de métastases chez les animaux exposés à l’halothane (37, 28±5, 08, P< 0, 0001) ou à l’isoflurane (28, 24±4, 07, P< 0.0014) que dans le groupe contrôle (12, 22± 1, 52).

Conclusion

L’exposition à l’halothane ou à l’isoflurane augmente le nombre de métastases pulmonaires chez de souris C57BI comparativement au groupe contrôle mais il n’y a aucune différence entre les animaux anesthésiés que soit à l’halothane ou à l’isoflurane.

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References

  1. Fidler IJ, Balch CM. The biology of cancer metastasis and implications for therapy. Curr Probl Surg 1987; 24: 137–209.
    Article Google Scholar
  2. Poste G, Fidler IJ. The pathogenesis of cancer metastasis. Nature 1980; 283: 139–46.
    Article PubMed CAS Google Scholar
  3. Talmadge JE, Fidler IJ. Cancer metastasis is selective or random depending on the parent tumour population. Nature 1982; 297: 593–4.
    Article PubMed CAS Google Scholar
  4. Talmadge JE, Fidler IJ. Enhanced metastatic potential of tumor cells harvested from spontaneous metastases of heterogeneous murine tumors. J Natl Cancer Inst 1982; 69: 975–80.
    PubMed CAS Google Scholar
  5. Moudgil GC, Wade AG. Anaesthesia and immunocompetence. Br J Anaesth 1976; 48: 31–9.
    Article PubMed CAS Google Scholar
  6. Moudgil GC. Update on anaesthesia and the immune response. Can Anaesth Soc J 1986; 33: S54–60.
    Article PubMed CAS Google Scholar
  7. Stevenson GW, Hall SC, Rudnick S, Seleny FL, Stevenson HC. The effect of anesthetic agents on the human immune response. Anesthesiology 1990; 72: 542–52.
    Article PubMed CAS Google Scholar
  8. Moudgil GC, Allan RB, Russell, RJ, Wilkinson PC. Inhibition, by anaesthetic agents, of human leucocyte locomotion towards chemical attractants. Br J Anaesth 1977; 49: 97–104.
    Article PubMed CAS Google Scholar
  9. Moudgil GC, Pandya, AR, Ludlow DJ. Influence of anaesthesia and surgery on neutrophil chemotaxis. Can Anaesth Soc J 1981; 28: 232–8.
    Article PubMed CAS Google Scholar
  10. Moudgil GC, Gordon J, Forrest JB. Comparative effects of volatile anaesthetic agents and nitrous oxide on human leucocyte chemotaxis in vitro. Can Anaesth Soc J 1984; 31: 631–7.
    Article PubMed CAS Google Scholar
  11. Moudgil GC. Effect of premedicants, intravenous anaesthetic agents and local anaesthetics on phagocytosis in vitro. Can Anaesth Soc J 1981; 28: 597–602.
    Article PubMed CAS Google Scholar
  12. Moudgil GC, Singal DP, Gordon J. Comparative effects of halothane and isoflurane anaesthesia on perioperative lymphocyte function. Anesthesiology 1992; 77: A354.
    Article Google Scholar
  13. Vose BM, Moudgil GC. Effect of surgery on tumourdirected leucocyte responses. BMJ 1975; 1: 56–8.
    Article PubMed CAS Google Scholar
  14. Shapiro J, Jersky J, Katzav S, Feldman M, Segal S. Anesthetic drugs accelerate the progression of postoperative metastases of mouse tumors. J Clin Invest 1981; 68: 678–85.
    Article PubMed CAS Google Scholar
  15. Lovett EJ III, Varani J, Lundy J. Suppressor cells and increased primary tumor growth rate induced by thiopental. J Surg Oncol 1983; 22: 26–32.
    Article PubMed CAS Google Scholar
  16. Varani J, Lovett EJ III, Lundy J. A model of tumor cell dormancy: effects of anesthesia and surgery. J Surg Oncol 1981; 17: 9–14.
    Article PubMed CAS Google Scholar
  17. Shirwadkar S, Blajchman MA, Frame B, Singal DP. Effect of allogeneic blood transfusion on solid tumor growth and pulmonary metastases in mice. J Cancer Res Clin Oncol 1992; 118: 176–80.
    Article PubMed CAS Google Scholar
  18. Eccles SA. Dormancy in experimental solid tumour systems.In: Stewart THM, Wheelock EF (Eds.). Cellular Immune Mechanisms and Tumor Dormancy. C Press Inc., 1992: 27–51.
  19. Woods GM, Griffiths DM. Reversible inhibition of natural killer cell activity by volatile anaesthetic agents in vitro. Br J Anaesth 1986; 58: 535–9.
    Article PubMed CAS Google Scholar
  20. Griffith CDM, Kamath MB. Effect of halothane and nitrous oxide anaesthesia on natural killer lymphocytes from patients with benign and malignant breast disease. Br J Anaesth 1986; 58: 540–3.
    Article PubMed CAS Google Scholar
  21. Whelan P, Morris PJ. Immunological responsiveness after transurethral resection of the prostate: general versus spinal anaesthetic. Clin Exp Immunol 1982; 48: 611–8.
    PubMed CAS Google Scholar
  22. Salo M, Eskola J, Nikoskelainen J. T and B lymphocyte function in anaesthetists. Acta Anaesthesiol Scand 1984; 28: 292–5.
    PubMed CAS Google Scholar
  23. Salo M. Effect of anaesthesia and open-heart surgery on lymphocyte responses to phytohaemagglutinin and concanavalin A. Acta Anaesthesiol Scand 1978; 22: 471–9.
    PubMed CAS Google Scholar
  24. Puri P, Brazil J, Reen DJ. Immunosuppressive effects of anesthesia and surgery in the new-born: I Shortterm effects. J Pediatr Surg 1984; 19: 823–8.
    Article PubMed CAS Google Scholar
  25. Mollitt DL, Marmer DJ, Steele RW. Age-dependent variation of lymphocyte function in the postoperative child. J Pediatr Surg 1986; 21: 633–5.
    Article PubMed CAS Google Scholar
  26. Riddle PR. Disturbed immune reactions following surgery. Br J Surg 1967; 54: 882–6.
    Article PubMed CAS Google Scholar
  27. Cullen BF, Duncan PG, Ray-Keil L. Inhibition of cellmediated cytotoxicity by halothane and nitrous oxide. Anesthesiology 1976; 44: 386–90.
    Article PubMed CAS Google Scholar
  28. Kumar S, Taylor G. Effect of surgery on lymphocytotoxicity against tumour cells. Lancet 1974; i: 1564–5.
    Article Google Scholar

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Authors and Affiliations

  1. Department of Anesthesiology, Tulane University and McMaster University Medical Centers, USA
    Girish C. Moudgil & Dharam P. Singal
  2. Department of Pathology, Tulane University and McMaster University Medical Centers, USA
    Girish C. Moudgil & Dharam P. Singal

Authors

  1. Girish C. Moudgil
  2. Dharam P. Singal

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Moudgil, G.C., Singal, D.P. Halothane and isoflurane enhance melanoma tumour metastasis in mice.Can J Anesth 44, 90–94 (1997). https://doi.org/10.1007/BF03014331

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