Plasmodium falciparum: a comparative analysis of the genetic diversity in malaria-mesoendemic areas of Brazil and Madagascar (original) (raw)

Abstract

For a better definition of the polymorphic features of Plasmodium falciparum parasite populations, the polymerase chain reaction (PCR) typing technique was used to investigate the genetic diversity and complexity of parasites harbored by acute P. falciparum carriers from three yet unexplored malaria-mesoendemic areas with different transmission levels: two localities in northwestern Brazil (Ariquemes and Porto Velho) and a village in Madagascar (Ankazobe). A total of 89 DNA samples were analyzed by amplification of polymorphic domains from genes encoding merozoite surface antigens 1 and 2 (MSP-1, MSP-2) and thrombospondin-related anonymous protein (TRAP) and by hybridization with allelic-family-specific probes or random-fragment-length polymorphism (RFLP). In all three localities, extensive polymorphism was observed for each marker, but the MSP-2 central repeat was the most diverse one. Similar levels of genetic diversity, allelic frequency, and infection complexity were observed in the two Brazilian localities, although the isolates had been sampled at 2-year intervals, suggesting the stability of the infecting parasite populations presenting in these regions of the Brazilian Amazon. Unexpectedly, although the entomologic inoculation rate was at least 3 times lower in Ankazobe than in the Brazilian areas, Malagasi samples appeared more complex than the Brazilian ones. The implications of these data with regard to parasite population-dynamics studies are discussed.

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Authors and Affiliations

1. WHO Collaborating Center for Research and Training in the Immunology of Parasitic Diseases, Departamento de Imunologia, Instituto Oswaldo Cruz/Fiocruz, Avenida Brasil 4365, Manguinhos, CEP 21045-900, Rio de Janeiro, Brazil e-mail: mffcruz@gene.dbbm.fiocruz.br Tel.: +55-21-2801486; Fax: +55-21-2801589, , , , , , BR
   S. Sallenave-Sales, C. T. Daniel-Ribeiro & M. F. Ferreira-da-Cruz
2. Unité de Parasitologie Biomédicale, Institut Pasteur, 75724 Paris Cedex 15, France, , , , , , FR
   P. Daubersies & P. Druilhe
3. Unité d'Immunologie Moléculaire des Parasites, Institut Pasteur, 75724 Paris Cedex 15, France, , , , , , FR
   O. Mercereau-Puijalon & H. Contamin
4. Unité de Recherches sur le Paludisme, Institut Pasteur de Madagascar, BP 1274 101 Antananarivo, Madagascar, , , , , , MG
   L. Rahimalala

Authors

1. S. Sallenave-Sales
2. P. Daubersies
3. O. Mercereau-Puijalon
4. L. Rahimalala
5. H. Contamin
6. P. Druilhe
7. C. T. Daniel-Ribeiro
8. M. F. Ferreira-da-Cruz

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Received: 5 September 1999 / Accepted: 1 October 1999

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Sallenave-Sales, S., Daubersies, P., Mercereau-Puijalon, O. et al. Plasmodium falciparum: a comparative analysis of the genetic diversity in malaria-mesoendemic areas of Brazil and Madagascar.Parasitol Res 86, 692–698 (2000). https://doi.org/10.1007/PL00008554

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• Issue date: July 2000
• DOI: https://doi.org/10.1007/PL00008554