Ribonuclease production of Rhizopus oryzae IFO 4697 under metal ion stress in liquid culture (original) (raw)

Abstract

Rhizopus oryzae IFO 4697 was found to produce intracellular ribonuclease (RNase), and its growth and activity could be regulated under selected metal ion stress. The addition of Fe2+, Mg2+ and Zn2+ to the SLSR medium was essential to growth and RNase production. Ca2+ and Mo6+ stimulated RNase production. It is concluded that the addition of 100 mg/ml Ca2+, 5 mg/ml Mo6+, 0.7 mg/ml Zn2+, 2 mg/ml Fe2+, and 49 mg/ml Mg2+ to the SLSR medium was the best condition for producing RNase in high specific activity (3780 U/mg protein). This result indicates that a metal ion-regulated liquid medium is an efficient culture method for RNase production.

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Authors and Affiliations

  1. Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, Hibikino, Wakamatsu-ku, Kitakyushu 808-0135, Japan Tel. +81-93-695-3289; Fax +81-93-695-3381 e-mail: morita@env.kitakyu-u.ac.jp, , , , , , JP
    H. Morita
  2. Department of Bioengineering, Yatsushiro National College of Technology, Yatsushiro, Japan, , , , , , JP
    M. Shiozawa
  3. Department of Food and Nutritions, Suzugamine Women's College, Hiroshima, Japan, , , , , , JP
    Y. Fujio

Authors

  1. H. Morita
  2. M. Shiozawa
  3. Y. Fujio

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Received: July 19, 2001 / Accepted: April 8, 2002

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Morita, H., Shiozawa, M. & Fujio, Y. Ribonuclease production of Rhizopus oryzae IFO 4697 under metal ion stress in liquid culture.Mycoscience 43, 0283–0287 (2002). https://doi.org/10.1007/s102670200042

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