Effects of activation of polymorphonuclear leukocytes on airway goblet cell mucin release in a co-culture system (original) (raw)

Abstract:

Objectives and Design: Effects of activated PMN on airway goblet cell mucin release were investigated using a co-culture system in which both tracheal surface epithelial (TSE) cells and PMN from hamsters were present.Materials and methods: TSE cells were metabolically labeled using 3H-glucosamine and chased in the presence of PMN under various experimental designs. PMN were obtained from the bronchoalveolar lavage fluid of hamsters following intratracheal instillation of E. coli endotoxin. The amount of 3H-mucin was measured by Sepharose CL-4B gel-filtration column chromatography.Results: (i) activation of 106 PMN by fMLP (0.1 μM) and cytochalasin B (0.1 μM) resulted in production of both the estrolytic ("elastolytic") activity and superoxide, (ii) activation of PMN in the co-culture stimulated mucin release from TSE cells followed by a significant degradation of the released mucins, both of which were blocked in a dose-dependent fashion by pretreatment with α1-protease inhibitor, and (iii) generation of varying concentrations of superoxide in the TSE cell culture did not affect mucin release from TSE cells.Conclusion: In the co-culture system, activation of PMN results in release and degradation of mucins, both of which are almost entirely accounted for by serine proteases but not other cellular products such as superoxide.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, 20 N. Pine St., Baltimore, MD 21201, USA, Fax: ++410 706 1702, e-mail: kkim@umaryland.edu , , , , , , US
    K. C. Kim
  2. Namsan Clinic, 184-34 Ku-Seo Dong, Keum-Jung Ku, Busan, Korea , , , , , , KR
    B. C. Lee
  3. Sovitj Scientific Consultant, 15410 SW Garibaldi Court, Beaverton, OR 97007, USA , , , , , , US
    S. Pou
  4. Department of Medicine, Pulmonary and Critical Care Section, Temple University School of Medicine, Philadelphia, PA, USA , , , , , , US
    D. Ciccolella

Authors

  1. K. C. Kim
  2. B. C. Lee
  3. S. Pou
  4. D. Ciccolella

Additional information

Received 9 August 2002; returned for revision 25 November 2002; accepted by I. Ahnfelt-Rønne 23 January 2003

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ID="*"Correspondence to: K. C. Kim.

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Kim, K., Lee, B., Pou, S. et al. Effects of activation of polymorphonuclear leukocytes on airway goblet cell mucin release in a co-culture system.Inflamm. res. 52, 258–262 (2003). https://doi.org/10.1007/s00011-003-1171-y

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