The JNK/AP1/ATF2 pathway is involved in H2O2-induced acetylcholinesterase expression during apoptosis (original) (raw)

Abstract.

We show that H2O2 increases acetylcholinesterase (AChE) expression via transcriptional activation through c-Jun N-terminal kinase (JNK), since the JNK inhibitor SP600125, but not the extracellular signal-regulated kinase (ERK) pathway inhibitor PD98059 or p38 kinase inhibitor SB203580, attenuated H2O2- induced AChE expression and its promoter activity. Over expression of hemagglutinin (HA)-JNK increases H2O2-induced AChE expression and its promoter activity, whereas the dominant negative mutant form of JNK suppressed H2O2-induced AChE expression and promoter activity. Mutation analysis indicates that the major response elements for JNK in the AChE promoter are the AP1–like element (TGAGTCT) site, located within the -1565/-1569 region of the AChE promoter, and the ATF2 element (CCACGTCA), within the -2185/-2177 region. The AP1-like element binds to the transcription factors, c-jun and ATF2, while the ATF2 element binds mainly ATF2. Taken together, our results strongly suggest that H2O2 induces AChE expression via the JNK/AP1/ATF2 signaling pathway.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, 320 YueYang Road, Shanghai, 200031, China
    J.-Y. Zhang, H. Jiang, W. Gao, J. Wu, K. Peng & X.-J. Zhang
  2. Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, NJ, 08854, USA
    Y.-F. Shi

Authors

  1. J.-Y. Zhang
    You can also search for this author inPubMed Google Scholar
  2. H. Jiang
    You can also search for this author inPubMed Google Scholar
  3. W. Gao
    You can also search for this author inPubMed Google Scholar
  4. J. Wu
    You can also search for this author inPubMed Google Scholar
  5. K. Peng
    You can also search for this author inPubMed Google Scholar
  6. Y.-F. Shi
    You can also search for this author inPubMed Google Scholar
  7. X.-J. Zhang
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toX.-J. Zhang.

Additional information

Received 25 January 2008; received after revision 14 February 2008; accepted 10 March 2008

Rights and permissions

About this article

Cite this article

Zhang, JY., Jiang, H., Gao, W. et al. The JNK/AP1/ATF2 pathway is involved in H2O2-induced acetylcholinesterase expression during apoptosis.Cell. Mol. Life Sci. 65, 1435–1445 (2008). https://doi.org/10.1007/s00018-008-8047-9

Download citation

Keywords.