Large ARF guanine nucleotide exchange factors in membrane trafficking (original) (raw)
Abstract.
In eukaryotic cells membrane compartments are connected through cargo-selective vesicle trafficking mediating the exchange of components between different organelles. This exchange is essential to maintain their structural integrity and specific composition. A fundamental regulatory step in vesicle formation is the activation of small ARF GTPases by exchanging their bound GDP for GTP, which is a prerequisite for ARF-mediated effector recruitment. Activation of ARFs is catalyzed by the characteristic SEC7 domain of guanine nucleotide exchange factors (ARF-GEFs), which are classified according to their additional protein domains.The only group of ARF-GEFs conserved in mammals, yeast and plants are the large ARF-GEFs. This review summarizes recent findings on the function of large ARF-GEFs, and the use of the inhibitor Brefeldin A as a potent tool in understanding membrane trafficking. Furthermore we highlight common themes and apparent differences in large ARF-GEF function between eukaryotic kingdoms.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
Author information
Authors and Affiliations
- Center of Molecular Biology of Plants, University of Tübingen, 72076, Tübingen, Germany
N. Anders & G. Jürgens
Authors
- N. Anders
You can also search for this author inPubMed Google Scholar - G. Jürgens
You can also search for this author inPubMed Google Scholar
Corresponding author
Correspondence toG. Jürgens.
Additional information
Received 25 April 2008; received after revision 26 May 2008; accepted 12 June 2008
Rights and permissions
About this article
Cite this article
Anders, N., Jürgens, G. Large ARF guanine nucleotide exchange factors in membrane trafficking.Cell. Mol. Life Sci. 65, 3433–3445 (2008). https://doi.org/10.1007/s00018-008-8227-7
- Published: 07 July 2008
- Issue Date: November 2008
- DOI: https://doi.org/10.1007/s00018-008-8227-7