Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants (original) (raw)

Abstract.

Many virus and transposon DNAs can integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.

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Authors and Affiliations

  1. Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei 11221 (Taiwan), Fax +886 2 28212880, e-mail: losj@ym.edu.tw, , , , , , TW
    E. K.-W. Hui & S. J. Lo
  2. Department of Medicine, School of Medicine, National Yang-Ming University (Taiwan), , , , , , TW
    P.-C. Wang

Authors

  1. E. K.-W. Hui
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  2. P.-C. Wang
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  3. S. J. Lo
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Additional information

Received 9 July 1998; received after revision 2 October 1998; accepted 7 October 1998

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Hui, EW., Wang, PC. & Lo, S. Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants.CMLS, Cell. Mol. Life Sci. 54, 1403–1411 (1998). https://doi.org/10.1007/s000180050262

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