Nitric oxide biosynthesis, nitric oxide synthase inhibitors and arginase competition for L-arginine utilization (original) (raw)

Abstract.

Nitric oxide (NO) is a recently discovered mediator produced by mammalian cells. It plays a key role in neurotransmission, control of blood pressure, and cellular defense mechanisms. Nitric oxide synthases (NOSs) catalyze the oxidation of L-arginine to NO and L-citrulline. NOSs are unique enzymes in that they possess on the same polypeptidic chain a reductase domain and an oxygenase domain closely related to cytochrome P450s. NO and superoxide formation as well as NOS stability are finely regulated by Ca2+/calmodulin interactions, by the cofactor tetrahydrobiopterin, and by substrate availability. Strong interactions between the L-arginine-metabolizing enzymes are clearly demonstrated by competition between NOSs and arginases for L-arginine utilization, and by potent inhibition of arginase activity by Nω-hydroxy-L-arginine, an intermediate in the L-arginine to NO pathway.

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Authors and Affiliations

  1. Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, URA 400 CNRS, 45 rue des Saints Pères, F-75270 Paris Cedex 06 (France), Fax +33 1 42 86 83 87, e-mail: boucher@bisance.citi2.fr, , , , , , FR
    J. L. Boucher & C. Moali
  2. ERS 571 CNRS, Bât. 430, Université Paris-Sud XI, F-91405 Orsay Cedex (France), , , , , , FR
    J. P. Tenu

Authors

  1. J. L. Boucher
  2. C. Moali
  3. J. P. Tenu

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Boucher, J., Moali, C. & Tenu, J. Nitric oxide biosynthesis, nitric oxide synthase inhibitors and arginase competition for L-arginine utilization.CMLS, Cell. Mol. Life Sci. 55, 1015–1028 (1999). https://doi.org/10.1007/s000180050352

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