Characterization of genetic variation in and phylogenetic relationships among diploid Aegilops species by AFLP: incongruity of chloroplast and nuclear data (original) (raw)

Abstract

Intra- and inter-specific genetic variation was investigated in seven diploid Aegilops species using the amplified fragment length polymorphism (AFLP) technique. Of the seven species, the cross-pollinating Aegilops speltoides and Aegilops mutica showed high levels of intraspecific variation whereas the remaining five self-pollinating species showed low levels. Aegilops bicornis, Aegilops searsii and Ae. speltoides formed one cluster in the dendrograms, while Aegilops caudata and Aegilops umbellulata formed another. Relationships among the species inferred were more consistent with the relationships inferred from studies of chromosome pairing in interspecific hybrids, and previous molecular phylogenetic reconstructions based on nuclear DNA, than they were with those based on molecular plasmon analysis, suggesting that the nuclear genome has evolved differently from the cytoplasmic genome in the genus Aegilops.

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Acknowledgements

We thank ICARDA’s Genebank for providing us with materials and facilities for use in experiments. We also thank Pr. J. Dvorak for his pertinent comments and Dr. N. T. Miyashita for providing us with the computer program used to estimate the value of nucleotide diversity. Thanks are also due to Dr. T. Blake and Dr. S. Grando, for their helpful comments and suggestions. This study was supported by Research Fellowships provided by the Japan Society for the Promotion of Young Scientists (T. Sasanuma). Contribution number 573 from the Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University.

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1. T. Sasanuma
   Present address: Kihara Institute for Biological Research, Yokohama City University, Maioka-cho 641-12, Totsuka-ku, 244-0813, Yokohama, Japan

Authors and Affiliations

1. Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan
   T. Sasanuma & T. R. Endo
2. Genetic Resources Unit, International Center for Agricultural Research in the Dry Areas, P. O. Box 5466, Aleppo, Syria
   K. Chabane & J. Valkoun

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1. T. Sasanuma
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2. K. Chabane
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3. T. R. Endo
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4. J. Valkoun
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Correspondence toT. Sasanuma.

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Communicated by J. Dvorak

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Sasanuma, T., Chabane, K., Endo, T.R. et al. Characterization of genetic variation in and phylogenetic relationships among diploid Aegilops species by AFLP: incongruity of chloroplast and nuclear data.Theor Appl Genet 108, 612–618 (2004). https://doi.org/10.1007/s00122-003-1485-8

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• Received: 08 May 2002
• Accepted: 28 August 2003
• Published: 29 October 2003
• Issue Date: February 2004
• DOI: https://doi.org/10.1007/s00122-003-1485-8

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