PCR-RFLP analysis of cpDNA in the genus Abies (original) (raw)

Abstract

We used PCR-RFLP analysis of the chloroplast DNA of the genus Abies (family Pinaceae), to determine if the method could be employed to detect inter-specific variation in this genus and to study how the variation was distributed in different regions of the genome. Ten different chloroplast DNA regions, consisting of coding and non-coding DNA sequences, were amplified with specific primers in ten different Abies taxa. The amplification products were digested with several restriction enzymes. The results showed that the chloroplast genome is highly variable in most of the investigated taxa and contains multiple variable regions that appear to be distributed throughout the whole genome. Species-diagnostic markers were found for four of the ten investigated species. Unexpectedly, intra-specific variation was also detected in four species. It is likely that further studies, including larger sample sizes and/or more powerful methods for the detection of chloroplast DNA variation, will reveal additional variation for this genus.

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Authors and Affiliations

  1. Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden Tel.:+46-090-786 62 91 Fax:+46-090-786 90 92 E-mail: alfred.szmidt@genfys.slu.se, , , , , , SE
    L. Parducci & A. E. Szmidt

Authors

  1. L. Parducci
  2. A. E. Szmidt

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Received: 2 September 1998 / Accepted: 17 September 1998

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Parducci, L., Szmidt, A. PCR-RFLP analysis of cpDNA in the genus Abies.Theor Appl Genet 98, 802–808 (1999). https://doi.org/10.1007/s001220051137

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