Mapping a high oleic acid mutation in winter oilseed rape (Brassica napus L.) (original) (raw)

Abstract

Two winter oilseed rape mutant lines, 7488 and 19661, with a high oleic (HO) acid content in the seed oil were characterized phenotypically. In both mutant lines the HO trait was monogenically inherited. Segregation analysis in an F2 population derived from a cross between 7488 and 19661 showed the two mutations to be allelic. From a comparison of seed, leaf and root fatty acid composition it was concluded that fad2, the endoplasmic oleic acid desaturase, is affected by the mutation. In a bulked segregant analysis three AFLP markers linked to this mutation were detected and localized on the genetic map of Brassica napus. The markers mapped near the locus of one copy of the fad2 gene in the rapeseed genome.

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Authors and Affiliations

  1. Institute of Agronomy and Plant Breeding, University of Göttingen; Von Siebold Strasse 8, 37075 Göttingen; Germany e-mail: aschier@gwdg.de, , , , , , DE
    A. Schierholt, H. C. Becker & W. Ecke

Authors

  1. A. Schierholt
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  2. H. C. Becker
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  3. W. Ecke
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Received: 16 February 2000 / Accepted: 28 March 2000

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Schierholt, A., Becker, H. & Ecke, W. Mapping a high oleic acid mutation in winter oilseed rape (Brassica napus L.).Theor Appl Genet 101, 897–901 (2000). https://doi.org/10.1007/s001220051559

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