Expression of peroxisome proliferator-activated receptor γ (PPARγ) in normal human pancreatic islet cells (original) (raw)

Abstract

Aims/hypothesis. Thiazolidinediones are reported to improve pancreatic islet morphology and beta-cell function in rodents, supporting the hypothesis of a direct action of thiazolidinediones on endocrine islet cells. In this study we examined the expression of the peroxisome proliferator-activated receptor γ, a nuclear receptor that is activated by naturally occurring fatty acids and synthetic thiazolidinediones, in normal human endocrine pancreatic cells. Methods. Human islets were isolated from pancreata harvested in ten brain-dead lean non-diabetic adult donors. We analysed the gene and protein expression of the human peroxisome proliferator-activated receptor γ and evaluated the effects of peroxisome proliferator-activated receptor γ agonist on insulin secretion in human islet preparations. Results. The RT-PCR carried out on total RNA from four distinct human islet preparations demonstrated the presence of peroxisome proliferator-activated receptor γ mRNA. Western blot analysis showed the consistent expression of peroxisome proliferator-activated receptor γ protein. Peroxisome proliferator-activated receptor γ was shown to be present in all three endocrine cell types studied (alpha, beta and delta cells) by immunohistochemistry. Conclusion/interpretation. We found that peroxisome proliferator-activated receptor γ is highly expressed in human islet endocrine cells, both at the mRNA and protein levels. These results support the hypothesis of a direct influence of peroxisome proliferator-activated receptor γ agonist on human pancreatic endocrine cells. [Diabetologia (2000) 43: 1165–1169]

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Authors and Affiliations

  1. UPRES 1048, University Hospital Centre of Lille, Lille, France, , , , , , FR
    M. Dubois, F. Pattou, J. Kerr-Conte, V. Gmyr, B. Vandewalle & J. Lefebvre
  2. Research Laboratory of Inflammatory Intestinal Disorders (CRI4U004B), University Hospital Centre of Lille, Lille, France, , , , , , FR
    P. Desreumaux
  3. Institut of Genetics and Molecular and Cellular Biology (IGBMC), CNRS, INSERM, ULP, Illkirch, France, , , , , , FR
    J. Auwerx & K. Schoonjans

Authors

  1. M. Dubois
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  2. F. Pattou
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  3. J. Kerr-Conte
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  4. V. Gmyr
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  5. B. Vandewalle
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  6. P. Desreumaux
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  7. J. Auwerx
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  8. K. Schoonjans
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  9. J. Lefebvre
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Received: 29 March 2000 and in revised form: 4 May 2000

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Dubois, M., Pattou, F., Kerr-Conte, J. et al. Expression of peroxisome proliferator-activated receptor γ (PPARγ) in normal human pancreatic islet cells.Diabetologia 43, 1165–1169 (2000). https://doi.org/10.1007/s001250051508

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