Accumulation of Copper in Gill, Liver, Spleen, Kidney and Muscle Tissues of Clarias gariepinus Exposed to the Metal Singly and in Mixture with Chitosan (original) (raw)

Abstract

Accumulation of copper (Cu), applied singly and in mixture with chitosan (CT), in gill, liver, spleen, kidney and muscle tissues of Clarias gariepinus was studied after exposing the fish to no Cu (control), 5 ppm Cu, 5 ppm Cu + 75 ppm CT mixture over 1, 7 and 15 days. Metal levels in tissues were determined using an ICP-AES spectrophotometer. No mortality was observed during the experiments. Highest accumulation of Cu was observed in liver while lowest accumulation was observed in muscle tissue. Exposure to Cu–CT mixture decreased Cu accumulation in liver at all exposure periods and in kidney on the 15th day compared to Cu alone. Exposure to Cu alone and Cu–CT mixture had no effect on Cu accumulation in spleen or muscle tissue. Copper accumulation increased in gill tissue compared to control when exposed to Cu alone at all exposure periods, and exposure to the Cu–CT mixture significantly increased Cu accumulation in this tissue at all exposure periods compared to Cu alone.

Access this article

Log in via an institution

Subscribe and save

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

Explore related subjects

Discover the latest articles, news and stories from top researchers in related subjects.

References

• Anderson DP, Siwicki AK, Rumsey GL (1995) Injection or immersion delivery of selected immunostimulants to trout demonstrate enhancement of nonspecific defense mechanisms and protective immunity. Dis Asian Aquac 11:413–426
  Google Scholar
• Bullock G, Blazer V, Tsukuda S, Summerfelt S (2000) Toxicity of acidified chitosan for cultured rainbow trout Oncorhynchus mykiss. Aquaculture 185:273–280
  Article CAS Google Scholar
• Cha SH, Lee JS, Song CB, Lee KJ, Jeon YJ (2008) Effects of chitosan-coated diet on improving water quality and innate immunity in the olive flounder, Paralichthys olivaceus. Aquaculture 278:110–118
  Article CAS Google Scholar
• Cicik B (2003) Bakır-çinko etkileşiminin sazan (Cyprinus carpio L.)’nın karaciğer, solungaç ve kas dokularındaki metal birikimi üzerine etkileri. Ekoloji 12(48):32–36
  CAS Google Scholar
• Dautremepuits C, Betoulle S, Paris-Palacios S, Vernet G (2004) Humoral immune factors modulated by copper and chitosan in healthy or parasitized carp (Cyprinus carpio L.) Ptychobothrium sp. (Cestoda). Aquat Toxicol 65:325–338
  Article Google Scholar
• Duran S, Erdem C (2014) Effects of sublethal concentration of copper, zinc and cadmium, applied singly and in mixture, on some hematological parameters of Oreochromis niloticus (L., 1758). Fresen Environ Bull 22(10A):2977–2980
  Google Scholar
• Esteban MA, Cuesta A, Ortuno J, Meseguer J (2001) Immunomodulatory effects of dietary intake of chitin in gilthead seabream (Sparus aurata L.) innate immune response. Fish Shellfish Immunol 11:303–315
  Article CAS Google Scholar
• Ferguson HW (1989) Systemic pathology of fish. Iowa State University Press, Ames
  Google Scholar
• Gopalakannan A, Arul V (2006) Immunomodulatory effects of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds. Aquaculture 255:179–187
  Article CAS Google Scholar
• Heath AG (1995) Water pollution and fish physiology. CRC Press, Florida
  Google Scholar
• McKay G, Blair HS, Findon A (1989) Equilibrium studies for the sorption of metal ions onto chitosan. Ind J Chem 28(2):356–360
  Google Scholar
• Muramoto S (1983) Elimination of copper from Cu contaminated fish by long-term exposure to EDTA and freshwater. J Environ Sci Health 18(3):455–461
  Google Scholar
• Nussey G, Van Vuren JHJ, Du Preez HH (1995) Effect of copper on the hematology and osmoregulation of the Mozambique Tilapia, Oreochromis mossambicus (Cichlidae). Comp Biochem Physiol 111C(3):369–380
  CAS Google Scholar
• Sakai M (1999) Current research status of fish immunostimulants. Aquaculture 172:63–92
  Article CAS Google Scholar
• Samarakoon KW, Cha SH, Lee JH, Jeon YJ (2013) The growth, innate immunity and protection against H2O2-induced oxidative damage of a chitosan-coated diet in the olive flounder Paralichthys olivaceus. Fish Aquat Sci 16(3):149–158
  CAS Google Scholar
• Sokal RR, Rohlf FJ (1995) Biometry: the principles and practice of statistics in biological research, 3rd edn. W.H. Freeman and Co., New York
  Google Scholar
• Tunçsoy M, Erdem C (2014) Accumulation of copper, zinc and cadmium in liver, gill and muscle tissues of Oreochromis niloticus exposed to these metals separately and in mixture. Fresen Environ Bull 23(5):1143–1149
  Google Scholar
• Tunçsoy M, Duran S, Ay Ö, Cicik B, Erdem C (2015) Effects of copper and lead applied singly and in mixture with chitosan on some sera parameters of Clarias gariepinus. Fresen Environ Bull 24(9a):3029–3034
  Google Scholar
• Zhou QF, Zhang JB, Fu JJ, Shi JB, Jiang GB (2008) Biomonitoring: an appealing tool for assessment of metal pollution in the aquatic ecosystem. Anal Chim Acta 606:135–150
  Article CAS Google Scholar

Download references

Acknowledgments

This work was supported by Çukurova University, Scientific Research Projects Coordination Unit.

Author information

Authors and Affiliations

1. Faculty of Science and Letters, Biology Department, Çukurova University, Adana, Turkey
   Mustafa Tunçsoy, Servet Duran & Cahit Erdem
2. Faculty of Fisheries, Mersin University, Yenişehir Campus, Yenişehir, Mersin, Turkey
   Özcan Ay & Bedii Cicik

Authors

1. Mustafa Tunçsoy
   You can also search for this author inPubMed Google Scholar
2. Servet Duran
   You can also search for this author inPubMed Google Scholar
3. Özcan Ay
   You can also search for this author inPubMed Google Scholar
4. Bedii Cicik
   You can also search for this author inPubMed Google Scholar
5. Cahit Erdem
   You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toMustafa Tunçsoy.

Rights and permissions

About this article

Cite this article

Tunçsoy, M., Duran, S., Ay, Ö. et al. Accumulation of Copper in Gill, Liver, Spleen, Kidney and Muscle Tissues of Clarias gariepinus Exposed to the Metal Singly and in Mixture with Chitosan.Bull Environ Contam Toxicol 97, 486–489 (2016). https://doi.org/10.1007/s00128-016-1900-x

Download citation

• Received: 03 January 2016
• Accepted: 02 August 2016
• Published: 08 August 2016
• Issue Date: October 2016
• DOI: https://doi.org/10.1007/s00128-016-1900-x

Keywords