Localization of RubisCO and sulfur in endosymbiotic bacteria of the gutless marine oligochaete Inanidrilus leukodermatus (Annelida) (original) (raw)

Abstract

The chemoautotrophic potential of the two co-occurring larger and smaller bacterial endosymbionts of the gutless marine oligochaete Inanidrilus leukodermatus was determined using immunocytochemistry. An antibody directed against the Form I of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), the key CO2-fixing enzyme of the Calvin–Benson cycle, consistently labeled the larger symbionts. Electron microscopic spectroscopy showed that the larger symbionts contained sulfur in intracellular globules and to a lesser degree in the cytoplasm. The presence of RubisCO and sulfur indicates that the larger endosymbionts of I. leukodermatus are chemoautotrophic sulfur-oxidizing bacteria. In contrast, no RubisCO or sulfur was detected in the smaller endosymbionts of this host.

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Authors and Affiliations

1. Zoological Institute and Zoological Museum, University of Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany Tel.: +49-40-428384234; Fax: +49-40-428383937 e-mail: jens.krieger@zoologie.uni-hamburg.de, , , , , , DE
   J. Krieger & O. Giere
2. Max-Planck-Institute for Marine Microbiology, Celsiusstraße 1, 28359 Bremen, Germany, , , , , , DE
   N. Dubilier

Authors

1. J. Krieger
2. O. Giere
3. N. Dubilier

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Received: 28 September 1999 / Accepted: 29 May 2000

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Krieger, J., Giere, O. & Dubilier, N. Localization of RubisCO and sulfur in endosymbiotic bacteria of the gutless marine oligochaete Inanidrilus leukodermatus (Annelida).Marine Biology 137, 239–244 (2000). https://doi.org/10.1007/s002270000355

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• Issue date: September 2000
• DOI: https://doi.org/10.1007/s002270000355

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